@inbook{65269ecde0e9466eb776c484fc758eea,
title = "Development of a huBLT Mouse Model to Study HCMV Latency, Reactivation, and Immune Response",
abstract = "Immunodeficient mice engrafted with human tissues provide a robust model for the in vivo investigation of human-restricted viruses such as human cytomegalovirus (HCMV). Several humanized mouse models have been developed and improved over the last 30 years. Here, we describe a protocol for the transplant of human hematopoietic stem cells with autologous fetal liver and thymic tissues into NOD.Cg-PrkdcscidIL2rγtm1Wjl mice to create a humanized bone marrow–liver–thymus model (huBLT) that can be infected with HCMV. The presence of human thymus allows the development of a functional human immune system, including HLA-restricted human T-cells and B-cells. Indeed, following infection, huBLT mice generate virus-specific CD4+ and CD8+ T-cell responses. Additionally, both HCMV-specific IgM and IgG B-cell responses can be detected. This huBLT model provides the first animal model to explore the adaptive human immune response to HCMV infection.",
keywords = "B-cell response, Hematopoietic progenitor cells, Human Cytomegalovirus, Humanized mice, T-cell response, Thymus, huBLT",
author = "Crawford, {Lindsey B.} and Patrizia Caposio",
note = "Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-1111-1_17",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "343--363",
booktitle = "Methods in Molecular Biology",
}