Determining cell-of-origin subtypes of diffuse large B-cell lymphoma using gene expression in formalin-fixed paraffin-embedded tissue

David W. Scott, George W. Wright, P. Mickey Williams, Chih Jian Lih, William Walsh, Elaine S. Jaffe, Andreas Rosenwald, Elias Campo, Wing C. Chan, Joseph M. Connors, Erlend B. Smeland, Anja Mottok, Rita M. Braziel, German Ott, Jan Delabie, Raymond R. Tubbs, James R. Cook, Dennis D. Weisenburger, Timothy C. Greiner, Betty J. Glinsmann-GibsonKai Fu, Louis M. Staudt, Randy D. Gascoyne, Lisa M. Rimsza

Research output: Contribution to journalArticle

271 Scopus citations

Abstract

The assignment of diffuse large B-cell lymphoma into cell-of-origin (COO) groups is becoming increasingly important with the emergence of novel therapies that have selective biological activity in germinal center B cell-like or activated B cell-like groups. The Lymphoma/Leukemia Molecular Profiling Project's Lymph2Cx assay is a parsimonious digital gene expression (NanoString)-based test for COO assignment in formalin-fixed paraffin-embedded tissue (FFPET). The 20-gene assay was trained using 51 FFPET biopsies; the locked assay was then validated using an independent cohort of 68 FFPET biopsies. Comparisons were made with COO assignment using the original COO model on matched frozen tissue. In the validation cohort, the assay was accurate, with only 1 case with definitive COO being incorrectly assigned, and robust, with >95% concordance of COO assignment between 2 independent laboratories. These qualities, along with the rapid turnaround time,make Lymph2Cx attractive for implementation in clinical trials and, ultimately, patient management.

Original languageEnglish (US)
Pages (from-to)1214-1217
Number of pages4
JournalBlood
Volume123
Issue number8
DOIs
StatePublished - Feb 20 2014
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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    Scott, D. W., Wright, G. W., Williams, P. M., Lih, C. J., Walsh, W., Jaffe, E. S., Rosenwald, A., Campo, E., Chan, W. C., Connors, J. M., Smeland, E. B., Mottok, A., Braziel, R. M., Ott, G., Delabie, J., Tubbs, R. R., Cook, J. R., Weisenburger, D. D., Greiner, T. C., ... Rimsza, L. M. (2014). Determining cell-of-origin subtypes of diffuse large B-cell lymphoma using gene expression in formalin-fixed paraffin-embedded tissue. Blood, 123(8), 1214-1217. https://doi.org/10.1182/blood-2013-11-536433