Determination of non-bilayer phospholipid arrangements and their antibodies in placentae and sera of patients with hypertensive disorders of pregnancy

Begona Campos, M. Chames, J. M. Lantry, J. P. Bill, A. Eis, D. Brockman, J. Neil, E. Tischner, J. Barton, C. Wong, S. Schwemberger, J. Cornelius, Leslie Myatt, I. Baeza, M. Hnat

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Studies suggest that preeclampsia (PE) originates in the placenta and is associated with deficient trophoblast invasion of spiral arteries. The direct cause remains unknown, but preeclampsia is often associated with circulating factors that can induce generalized endothelial dysfunction. Antiphospholipid antibodies (APA) in circulation are also associated with vascular diseases. Although the quantification of APA is not currently used as a prognostic of the risk of PE, studies suggest that thrombophilias play a role in PE pathogenesis. In fact, the pathology of placentae from PE and Antiphospholipid syndrome patients is similar; atherosis, thrombosis and infarction, and endothelium activation represent the pathological mechanisms. We identified a new antibody which recognizes non-bilayer phospholipid arrangements (NPA) in membrane models and in cell membranes in vivo, and which triggered an autoimmune-like disease in mice. We evaluated the presence of NPA in the placentae and in sera, and whether NPA induced NPA antibodies in patients with hypertensive disorders of pregnancy (HDP). Results showed increased levels of NPA in the syncytiotrophoblast, extravillous cytotrophoblast, syncytial knots and the amnion epithelial cell membranes of the placenta, as well as increases in NPA and NPA antibodies in sera from HDP patients, when compared with controls. This suggests that NPA derived from placenta could be one of multiple factors associated with pregnancy pathologies.

Original languageEnglish (US)
Pages (from-to)215-224
Number of pages10
JournalPlacenta
Volume27
Issue number2-3
DOIs
StatePublished - Feb 2006
Externally publishedYes

Fingerprint

Placenta
Phospholipids
Pre-Eclampsia
Pregnancy
Antibodies
Serum
Trophoblasts
Antiphospholipid Antibodies
Cell Membrane
Pathology
Amnion
Antiphospholipid Syndrome
Vascular Diseases
Infarction
Autoimmune Diseases
Endothelium
Thrombosis
Arteries
Epithelial Cells
Membranes

Keywords

  • Antiphospholipid antibodies
  • Non-bilayer phospholipid arrangements
  • Preeclampsia
  • Pregnancy

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Determination of non-bilayer phospholipid arrangements and their antibodies in placentae and sera of patients with hypertensive disorders of pregnancy. / Campos, Begona; Chames, M.; Lantry, J. M.; Bill, J. P.; Eis, A.; Brockman, D.; Neil, J.; Tischner, E.; Barton, J.; Wong, C.; Schwemberger, S.; Cornelius, J.; Myatt, Leslie; Baeza, I.; Hnat, M.

In: Placenta, Vol. 27, No. 2-3, 02.2006, p. 215-224.

Research output: Contribution to journalArticle

Campos, B, Chames, M, Lantry, JM, Bill, JP, Eis, A, Brockman, D, Neil, J, Tischner, E, Barton, J, Wong, C, Schwemberger, S, Cornelius, J, Myatt, L, Baeza, I & Hnat, M 2006, 'Determination of non-bilayer phospholipid arrangements and their antibodies in placentae and sera of patients with hypertensive disorders of pregnancy', Placenta, vol. 27, no. 2-3, pp. 215-224. https://doi.org/10.1016/j.placenta.2005.01.010
Campos, Begona ; Chames, M. ; Lantry, J. M. ; Bill, J. P. ; Eis, A. ; Brockman, D. ; Neil, J. ; Tischner, E. ; Barton, J. ; Wong, C. ; Schwemberger, S. ; Cornelius, J. ; Myatt, Leslie ; Baeza, I. ; Hnat, M. / Determination of non-bilayer phospholipid arrangements and their antibodies in placentae and sera of patients with hypertensive disorders of pregnancy. In: Placenta. 2006 ; Vol. 27, No. 2-3. pp. 215-224.
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AU - Chames, M.

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AU - Bill, J. P.

AU - Eis, A.

AU - Brockman, D.

AU - Neil, J.

AU - Tischner, E.

AU - Barton, J.

AU - Wong, C.

AU - Schwemberger, S.

AU - Cornelius, J.

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AU - Baeza, I.

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