Determination of lactogenic activity in the serum of the squirrel monkey (Saimiri boliviensis) using the Nb2 lymphoma bioassay

M. E. Thompson, L. A. Macmillan, Cynthia Bethea, L. E. Williams, C. R. Abee, J. G. Scammell

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Determination of squirrel monkey prolactin by immunoassay has been hampered by the lack of antiserum specific to prolactin from this species. As an alternate method, we have investigated whether the Nb2 lymphoma bioassay could be adapted for routine measurement of the lactogenic activity of samples of squirrel monkey serum. The growth of the Nb2 cells is absolutely dependent on the presence of lactogens in the culture medium. The cells were maintained in Fisher's medium supplemented with 10% horse serum, 10% fetal calf serum (FCS), and 10−4M β‐mercaptoethanol. For each assay, the cells were plated at an initial density of 1 × 105 cells/ml in 22‐mm 12‐well dishes in the above medium, but devoid of FCS. Serum samples were heated to 56°C for 20 minutes to abolish the unusually high cytolytic complement activity of squirrel monkey serum and were incubated for 72 hours with Nb2 cells at serial dilutions from 1/40 to 1/2,560. Growth curves were generated with pooled samples of squirrel monkey serum, and the level of lactogenic activity was estimated using a calibration growth curve generated with known concentrations of purified rhesus monkey prolactin standard. We have found that the Nb2 lymphoma bioassay provides a sensitive and adaptable means for determination of lactogenic activity in the serum of the squirrel monkey.

    Original languageEnglish (US)
    Pages (from-to)175-181
    Number of pages7
    JournalAmerican Journal of Primatology
    Volume19
    Issue number3
    DOIs
    StatePublished - 1989

    Fingerprint

    Saimiri
    blood serum
    lymphoma
    bioassay
    serum
    bioassays
    prolactin
    fetal bovine serum
    growth curve
    cells
    Macaca mulatta
    immunoassays
    sampling
    antiserum
    cell growth
    immunoassay
    complement
    calibration
    horse
    culture media

    Keywords

    • complement
    • cortisol
    • Nb2 bioassay
    • squirrel monkey

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Animal Science and Zoology

    Cite this

    Determination of lactogenic activity in the serum of the squirrel monkey (Saimiri boliviensis) using the Nb2 lymphoma bioassay. / Thompson, M. E.; Macmillan, L. A.; Bethea, Cynthia; Williams, L. E.; Abee, C. R.; Scammell, J. G.

    In: American Journal of Primatology, Vol. 19, No. 3, 1989, p. 175-181.

    Research output: Contribution to journalArticle

    Thompson, M. E. ; Macmillan, L. A. ; Bethea, Cynthia ; Williams, L. E. ; Abee, C. R. ; Scammell, J. G. / Determination of lactogenic activity in the serum of the squirrel monkey (Saimiri boliviensis) using the Nb2 lymphoma bioassay. In: American Journal of Primatology. 1989 ; Vol. 19, No. 3. pp. 175-181.
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