Determination of cerebrospinal fluid production rate using a push-pull perfusion procedure in the conscious rat

V. Baudrie, Jean-Baptiste Roullet, Y. Goureau, F. Chaouloff, JL Elghozi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The inulin dilution technique was used to determine the cerebrospinal fluid (CSF) production rate by means of a push-pull cannula implanted into a lateral ventricle. Artificial CSF containing trace amount of 3H-inulin was perfused for 2 h in conscious rats. 3H-inulin in the effluent reached a plateau level depending on the CSF production rate. The control lateroventricular CSF production was 0.98 μl/min. Production was reduced to 0.34 μl/min during a perfusion with acetazolamide (1 mM), a carbonic anhydrase inhibitor.

Original languageEnglish (US)
Pages (from-to)269-274
Number of pages6
JournalFundamental and Clinical Pharmacology
Volume4
Issue number3
StatePublished - 1990
Externally publishedYes

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Inulin
Cerebrospinal Fluid
Perfusion
Carbonic Anhydrase Inhibitors
Indicator Dilution Techniques
Acetazolamide
Lateral Ventricles

Keywords

  • cerebrospinal fluid (CSF)
  • conscious rat
  • inulin
  • lateral ventricle
  • push-pull perfusion

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

Cite this

Determination of cerebrospinal fluid production rate using a push-pull perfusion procedure in the conscious rat. / Baudrie, V.; Roullet, Jean-Baptiste; Goureau, Y.; Chaouloff, F.; Elghozi, JL.

In: Fundamental and Clinical Pharmacology, Vol. 4, No. 3, 1990, p. 269-274.

Research output: Contribution to journalArticle

Baudrie, V, Roullet, J-B, Goureau, Y, Chaouloff, F & Elghozi, JL 1990, 'Determination of cerebrospinal fluid production rate using a push-pull perfusion procedure in the conscious rat', Fundamental and Clinical Pharmacology, vol. 4, no. 3, pp. 269-274.
Baudrie, V. ; Roullet, Jean-Baptiste ; Goureau, Y. ; Chaouloff, F. ; Elghozi, JL. / Determination of cerebrospinal fluid production rate using a push-pull perfusion procedure in the conscious rat. In: Fundamental and Clinical Pharmacology. 1990 ; Vol. 4, No. 3. pp. 269-274.
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