Determination of cerebrospinal fluid production rate using a push‐pull perfusion procedure in the conscious rat

V. Baudrie; JB Roullet; Y. Goureau; F. Chaouloff; JL Elghozi

doi:10.1111/j.1472-8206.1990.tb00494.x

Determination of cerebrospinal fluid production rate using a push‐pull perfusion procedure in the conscious rat

V. Baudrie, JB Roullet, Y. Goureau, F. Chaouloff, JL Elghozi

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Summary— The inulin dilution technique was used to determine the cerebrospinal fluid (CSF) production rate by means of a push‐pull cannula implanted into a lateral ventricle. Artificial CSF containing trace amount of ³H‐inulin was perfused for 2 h in conscious rats. ³H‐inulin in the effluent reached a plateau level depending on the CSF production rate. The control lateroventricular CSF production was 0.98 μl/min. Production was reduced to 0.34 μl/min during a perfusion with acetazolamide (l mM), a carbonic anhydrase inhibitor. 1990 Société Française de Pharmacologie et de Thérapeutique

Original language	English (US)
Pages (from-to)	269-274
Number of pages	6
Journal	Fundamental & Clinical Pharmacology
Volume	4
Issue number	3
DOIs	https://doi.org/10.1111/j.1472-8206.1990.tb00494.x
State	Published - 1990
Externally published	Yes

Keywords

cerebrospinal fluid (CSF)
conscious rat
inulin
lateral ventricle
push‐pull perfusion

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)

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10.1111/j.1472-8206.1990.tb00494.x

Cite this

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abstract = "Summary— The inulin dilution technique was used to determine the cerebrospinal fluid (CSF) production rate by means of a push‐pull cannula implanted into a lateral ventricle. Artificial CSF containing trace amount of 3H‐inulin was perfused for 2 h in conscious rats. 3H‐inulin in the effluent reached a plateau level depending on the CSF production rate. The control lateroventricular CSF production was 0.98 μl/min. Production was reduced to 0.34 μl/min during a perfusion with acetazolamide (l mM), a carbonic anhydrase inhibitor. 1990 Soci{\'e}t{\'e} Fran{\c c}aise de Pharmacologie et de Th{\'e}rapeutique",

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AU - Baudrie, V.

AU - Roullet, JB

AU - Goureau, Y.

AU - Chaouloff, F.

AU - Elghozi, JL

PY - 1990

Y1 - 1990

N2 - Summary— The inulin dilution technique was used to determine the cerebrospinal fluid (CSF) production rate by means of a push‐pull cannula implanted into a lateral ventricle. Artificial CSF containing trace amount of 3H‐inulin was perfused for 2 h in conscious rats. 3H‐inulin in the effluent reached a plateau level depending on the CSF production rate. The control lateroventricular CSF production was 0.98 μl/min. Production was reduced to 0.34 μl/min during a perfusion with acetazolamide (l mM), a carbonic anhydrase inhibitor. 1990 Société Française de Pharmacologie et de Thérapeutique

AB - Summary— The inulin dilution technique was used to determine the cerebrospinal fluid (CSF) production rate by means of a push‐pull cannula implanted into a lateral ventricle. Artificial CSF containing trace amount of 3H‐inulin was perfused for 2 h in conscious rats. 3H‐inulin in the effluent reached a plateau level depending on the CSF production rate. The control lateroventricular CSF production was 0.98 μl/min. Production was reduced to 0.34 μl/min during a perfusion with acetazolamide (l mM), a carbonic anhydrase inhibitor. 1990 Société Française de Pharmacologie et de Thérapeutique

KW - cerebrospinal fluid (CSF)

KW - conscious rat

KW - inulin

KW - lateral ventricle

KW - push‐pull perfusion

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Determination of cerebrospinal fluid production rate using a push‐pull perfusion procedure in the conscious rat

Abstract

Keywords

ASJC Scopus subject areas

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