Determination of antigen-specific memory/effector CD4+ T cell frequencies by flow cytometry. Evidence for a novel, antigen-specific homeostatic mechanism in HIV-associated immunodeficiency

Shar L. Waldrop, Christine J. Pitcher, Dolores M. Peterson, Vernon C. Maino, Louis Picker

Research output: Contribution to journalArticle

472 Scopus citations

Abstract

The highly regulated secretion of effector cytokines by CD4+ T cells plays a critical role in immune protection against pathogens such as cytomegalovirus. Here, we directly compare the frequency and functional characteristics of cytomegalovirus-specific CD4+ memory/effector T cells in normal and HIV+ subjects using a novel, highly efficient multiparameter flow cytometric assay that detects the rapid intracellular accumulation of cytokine(s) after short-term (6 h) in vitro antigen stimulation. Responses in this assay correlate precisely with independent measures of sensitization history (e.g., seroreactivity), and allow the simultaneous assessment of multiple cytokines in single effector T cells. Healthy HIV- individuals manifested an average of 0.71, 0.72, 0.38, and 0.06% CD4+ T cells responding to cytomegalovirus with γ-IFN, TNF-α, IL-2, and IL-4 production, respectively, with the simultaneous production of γ-IFN, TNF-α, and IL-2 being the most common effector phenotype. Significantly, overall cytomegalovirus-specific CD4+ effector frequencies were markedly higher among 40% of HIV-+ subjects (2.7-8.0%), and demonstrated a predominately polarized γ-IFN+/TNF-α+/IL-2-/IL-4- phenotype. In contrast, CD4+ effector frequencies for heterologous, nonubiquitous viruses such as the mumps virus were low or absent in the HIV+ group. These data suggest the existence of homeostatic mechanisms in HIV disease that selectively preserve memory T cell populations reactive with ubiquitous pathogens such as cytomegalovirus-likely at the expense of T cell memory to more sporadically encountered infectious agents.

Original languageEnglish (US)
Pages (from-to)1739-1750
Number of pages12
JournalJournal of Clinical Investigation
Volume99
Issue number7
Publication statusPublished - Apr 1 1997
Externally publishedYes

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Keywords

  • cytokines
  • flow cytometry
  • HIV immunodeficiency
  • T cells

ASJC Scopus subject areas

  • Medicine(all)

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