Determination of acrylamide in nerve tissue homogenates by electron-capture gas chromatography

C. F. Poole, W. F. Sye, A. Zlatkis, Peter Spencer

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Acrylamide in biological samples can be determined by gas chromatography with electron-capture detection after conversion to its 2,3-dibromopropionamide derivative. The derivatization is carried out in aqueous solution, plasma or tissue homogenates by ionic bromination and the reaction conditions and sample clean-up are described. The detection limit corresponds to 9.5 {star, open} 10-12 g of acrylamide on column or 8.4 {star, open} 10-9 g in the final biological extract (0.5 ml). The overall recovery of acrylamide spiked samples at the nanogram level exceeds 80%. It was found that the accumulation of free acrylamide in the science nerve region of rats intoxicated with acrylamide was less than 2-8 ppm.

Original languageEnglish (US)
Pages (from-to)239-245
Number of pages7
JournalJournal of Chromatography A
Volume217
Issue numberC
DOIs
StatePublished - Nov 6 1981
Externally publishedYes

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Nerve Tissue
Acrylamide
Gas chromatography
Gas Chromatography
Electrons
Tissue
Stars
Halogenation
Limit of Detection
Rats
Derivatives
Plasmas
Recovery

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Determination of acrylamide in nerve tissue homogenates by electron-capture gas chromatography. / Poole, C. F.; Sye, W. F.; Zlatkis, A.; Spencer, Peter.

In: Journal of Chromatography A, Vol. 217, No. C, 06.11.1981, p. 239-245.

Research output: Contribution to journalArticle

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