Detection of K-ras mutations in pancreatic and hepatic neoplasms by non-isotopic mismatched polymerase chain reaction

Philip Stork, Massimo Loda, Silvano Bosari, Brian Wiley, Karin Poppenhusen, Hubert Wolfe

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Mutations within codon 12 leading to activation of Kirsten-ras (K-ras) genes occur in a wide variety of human tumors, but have been reported most frequently in pancreatic carcinomas. We studied twenty-four paraffinembedded pancreatic and hepatic tumors and two colon carcinoma cell lines with a rapid and simple approach that exploits allele-specific amplification of genomic DNA in a polymerase chain reaction (PCR). We extend the utility of this technique, which is dependent on an exact match at the 3′ nucleotide between synthetic oligonucleotides and template DNA, to analyse paraffinembedded tumor samples for the presence of point mutations at the first and second base of codon 12 of the K-ras gene. The PCR mismatch amplification technique demonstrated a 66% incidence of K-ras mutations at codon 12 in the group of pancreatic neoplasms as a whole. The percentage of mutations varied only slightly in the pancreatic cancer subcategories: 75% in ampullary, 66% in bile duct and 57% in the ductal adenocarcinomas. One islet cell carcinoma and normal tissues adjacent to the tumors revealed wild-type alleles only. One hepatoblastoma and one of six hepatocellular carcinomas also had codon 12 mutations. The PCR mismatch is a sensitive and rapid method that may be useful in screening neoplasms for K-ras point mutation and can be applied to archival material. This application allows a retrospective analyses of a wide range of pathological specimens to determine the role of K-ras mutations in human tumorigenesis.

Original languageEnglish (US)
Pages (from-to)857-862
Number of pages6
JournalOncogene
Volume6
Issue number5
StatePublished - May 1991
Externally publishedYes

Fingerprint

Liver Neoplasms
Pancreatic Neoplasms
Codon
Polymerase Chain Reaction
Mutation
ras Genes
Neoplasms
Point Mutation
Islet Cell Carcinoma
Alleles
Hepatoblastoma
DNA
Bile Ducts
Oligonucleotides
Hepatocellular Carcinoma
Colon
Carcinogenesis
Adenocarcinoma
Nucleotides
Carcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Stork, P., Loda, M., Bosari, S., Wiley, B., Poppenhusen, K., & Wolfe, H. (1991). Detection of K-ras mutations in pancreatic and hepatic neoplasms by non-isotopic mismatched polymerase chain reaction. Oncogene, 6(5), 857-862.

Detection of K-ras mutations in pancreatic and hepatic neoplasms by non-isotopic mismatched polymerase chain reaction. / Stork, Philip; Loda, Massimo; Bosari, Silvano; Wiley, Brian; Poppenhusen, Karin; Wolfe, Hubert.

In: Oncogene, Vol. 6, No. 5, 05.1991, p. 857-862.

Research output: Contribution to journalArticle

Stork, P, Loda, M, Bosari, S, Wiley, B, Poppenhusen, K & Wolfe, H 1991, 'Detection of K-ras mutations in pancreatic and hepatic neoplasms by non-isotopic mismatched polymerase chain reaction', Oncogene, vol. 6, no. 5, pp. 857-862.
Stork, Philip ; Loda, Massimo ; Bosari, Silvano ; Wiley, Brian ; Poppenhusen, Karin ; Wolfe, Hubert. / Detection of K-ras mutations in pancreatic and hepatic neoplasms by non-isotopic mismatched polymerase chain reaction. In: Oncogene. 1991 ; Vol. 6, No. 5. pp. 857-862.
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