Detection of interleukin-6 and interleukin-1 production in human thyroid epithelial cells by non-radioactive in situ hybridization and immunohistochemical methods

R. Q H Zheng, E. Abney, Cong-Qiu Chu, M. Field, B. Grubeck-Loebenstein, R. N. Maini, M. Feldmann

Research output: Contribution to journalArticle

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Abstract

Human endocrine thyroid epithelial cells have been described to produce cytokines in vitro. In order to determine whether they do so in vivo during thyroiditis, parallel studies on mRNA expression with a non-radioactive in situ hybridization technique and immunohistochemical detection for the protein were performed on frozen sections of thyroid samples from autoimmune thyroiditis (Graves' disease and Hashimoto's thyroiditis), non-toxic goitre and normal thyroid tissue. cDNA probes were sulphonated and their hybridization with mRNA was detected with a sulphonyl-specific monoclonal antibody. This signal was amplified and visualized with the alkaline phosphatase-anti-alkaline phosphatase (APAAP) system. The protein products were detected with immuno-purified rabbit F(ab')2 antibody fragments recognizing recombinant human cytokines, visualized by the immuno-peroxidase technique. Each sample was studied at the two levels. Both interleukin-6 mRNA and protein were found in the endocrine cells. There was no obvious difference between autoimmune thyroiditis and non-toxic goitre. However, normal thyroid epithelial cells produced less interleukin-6. Interleukin-1α mRNA and its protein were found in epithelial cells from Hashimoto's thyroiditis samples, but not in the others, except one Graves' disease sample, in which only mRNA was detected. Interleukin-1β was not detected in these cells, its mRNA was only found in one of the Graves' disease samples. These cytokines were also detected in some infiltrating cells.

Original languageEnglish (US)
Pages (from-to)314-319
Number of pages6
JournalClinical and Experimental Immunology
Volume83
Issue number2
StatePublished - 1991
Externally publishedYes

Fingerprint

Interleukin-1
In Situ Hybridization
Interleukin-6
Messenger RNA
Graves Disease
Autoimmune Thyroiditis
Hashimoto Disease
Goiter
Cytokines
Alkaline Phosphatase
Thyroid Gland
Proteins
Thyroiditis
Immunoglobulin Fragments
Endocrine Cells
Frozen Sections
Peroxidase
Thyroid Epithelial Cells
Complementary DNA
Epithelial Cells

Keywords

  • Cytokines
  • Immunohistochemistry
  • Non-radioactive in situ hybridization
  • Thyroid epithelial cells

ASJC Scopus subject areas

  • Immunology

Cite this

Zheng, R. Q. H., Abney, E., Chu, C-Q., Field, M., Grubeck-Loebenstein, B., Maini, R. N., & Feldmann, M. (1991). Detection of interleukin-6 and interleukin-1 production in human thyroid epithelial cells by non-radioactive in situ hybridization and immunohistochemical methods. Clinical and Experimental Immunology, 83(2), 314-319.

Detection of interleukin-6 and interleukin-1 production in human thyroid epithelial cells by non-radioactive in situ hybridization and immunohistochemical methods. / Zheng, R. Q H; Abney, E.; Chu, Cong-Qiu; Field, M.; Grubeck-Loebenstein, B.; Maini, R. N.; Feldmann, M.

In: Clinical and Experimental Immunology, Vol. 83, No. 2, 1991, p. 314-319.

Research output: Contribution to journalArticle

Zheng, R. Q H ; Abney, E. ; Chu, Cong-Qiu ; Field, M. ; Grubeck-Loebenstein, B. ; Maini, R. N. ; Feldmann, M. / Detection of interleukin-6 and interleukin-1 production in human thyroid epithelial cells by non-radioactive in situ hybridization and immunohistochemical methods. In: Clinical and Experimental Immunology. 1991 ; Vol. 83, No. 2. pp. 314-319.
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