Detection of in vivo production of tumour necrosis factor-alpha by human thyroid epithelial cells

R. Q.H. Zheng, E. R. Abney, C. Q. Chu, M. Field, R. N. Maini, J. R. Lamb, M. Feldmann

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Abstract

We have established previously that human thyroid epithelial cells (TEC) from patients with autoimmune thyroiditis are able to synthesize cytokines, such as interleukin-1 (IL-1) and interleukin-6 (IL-6). This paper examines TEC in sections from autoimmune thyroiditis for the in vivo production of tumour necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ) using the combined techniques of in situ hybridization and immunohistochemistry. Thyroid tissue from patients with Graves' disease, Hashimoto's disease and non-toxic goitre was examined and both mRNA and the protein of TNF-α were detected in TEC on frozen sections. Representative figures of only Grave's samples are illustrated in this paper. In contrast, using the same methods, IFN-γ was detected only in the infiltrating cells and not in TEC of thyroid tissue from the patients.

Original languageEnglish (US)
Pages (from-to)456-462
Number of pages7
JournalImmunology
Volume75
Issue number3
StatePublished - Jan 1 1992

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Zheng, R. Q. H., Abney, E. R., Chu, C. Q., Field, M., Maini, R. N., Lamb, J. R., & Feldmann, M. (1992). Detection of in vivo production of tumour necrosis factor-alpha by human thyroid epithelial cells. Immunology, 75(3), 456-462.