Detection of in vivo production of tumour necrosis factor-alpha by human thyroid epithelial cells

R. Q H Zheng, E. R. Abney, Cong-Qiu Chu, M. Field, R. N. Maini, J. R. Lamb, M. Feldmann

Research output: Contribution to journalArticle

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Abstract

We have established previously that human thyroid epithelial cells (TEC) from patients with autoimmune thyroiditis are able to synthesize cytokines, such as interleukin-1 (IL-1) and interleukin-6 (IL-6). This paper examines TEC in sections from autoimmune thyroiditis for the in vivo production of tumour necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ) using the combined techniques of in situ hybridization and immunohistochemistry. Thyroid tissue from patients with Graves' disease, Hashimoto's disease and non-toxic goitre was examined and both mRNA and the protein of TNF-α were detected in TEC on frozen sections. Representative figures of only Grave's samples are illustrated in this paper. In contrast, using the same methods, IFN-γ was detected only in the infiltrating cells and not in TEC of thyroid tissue from the patients.

Original languageEnglish (US)
Pages (from-to)456-462
Number of pages7
JournalImmunology
Volume75
Issue number3
StatePublished - 1992
Externally publishedYes

Fingerprint

Tumor Necrosis Factor-alpha
Autoimmune Thyroiditis
Interferon-gamma
Thyroid Gland
Hashimoto Disease
Graves Disease
Goiter
Frozen Sections
Interleukin-1
Interferon-alpha
In Situ Hybridization
Interleukin-6
Immunohistochemistry
Cytokines
Messenger RNA
Thyroid Epithelial Cells
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Zheng, R. Q. H., Abney, E. R., Chu, C-Q., Field, M., Maini, R. N., Lamb, J. R., & Feldmann, M. (1992). Detection of in vivo production of tumour necrosis factor-alpha by human thyroid epithelial cells. Immunology, 75(3), 456-462.

Detection of in vivo production of tumour necrosis factor-alpha by human thyroid epithelial cells. / Zheng, R. Q H; Abney, E. R.; Chu, Cong-Qiu; Field, M.; Maini, R. N.; Lamb, J. R.; Feldmann, M.

In: Immunology, Vol. 75, No. 3, 1992, p. 456-462.

Research output: Contribution to journalArticle

Zheng, RQH, Abney, ER, Chu, C-Q, Field, M, Maini, RN, Lamb, JR & Feldmann, M 1992, 'Detection of in vivo production of tumour necrosis factor-alpha by human thyroid epithelial cells', Immunology, vol. 75, no. 3, pp. 456-462.
Zheng RQH, Abney ER, Chu C-Q, Field M, Maini RN, Lamb JR et al. Detection of in vivo production of tumour necrosis factor-alpha by human thyroid epithelial cells. Immunology. 1992;75(3):456-462.
Zheng, R. Q H ; Abney, E. R. ; Chu, Cong-Qiu ; Field, M. ; Maini, R. N. ; Lamb, J. R. ; Feldmann, M. / Detection of in vivo production of tumour necrosis factor-alpha by human thyroid epithelial cells. In: Immunology. 1992 ; Vol. 75, No. 3. pp. 456-462.
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