Detection of differential gene expression in brown adipose tissue of hibernating arctic ground squirrels with mouse microarrays

Jun Yan, Adlai Burman, Calen Nichols, Linda Alila, Louise C. Showe, Michael K. Showe, Bert Boyer, Brian M. Barnes, Thomas G. Marr

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Hibernation is an energy-saving strategy adopted by a wide range of mammals to survive highly seasonal or unpredictable environments. Arctic ground squirrels living in Alaska provide an extreme example, with 6- to 9-mo-long hibernation seasons when body temperature alternates between levels near 0°C during torpor and 37°C during arousal episodes. Heat production during hibernation is provided, in part, by nonshivering thermogenesis that occurs in large deposits of brown adipose tissue (BAT). BAT is active at tissue temperatures from 0 to 37°C during rewarming and continuously at near 0°C during torpor in subfreezing conditions. Despite its crucial role in hibernation, the global gene expression patterns in BAT during hibernation compared with the nonhibernation season remain largely unknown. We report a large-scale study of differential gene expression in BAT between winter hibernating and summer active arctic ground squirrels using mouse microarrays. Selected differentially expressed genes identified on the arrays were validated by quantitative real-time PCR using ground squirrel specific primers. Our results show that the mRNA levels of the genes involved in nearly every step of the biochemical pathway leading to nonshivering thermogenesis are significantly increased in BAT during hibernation, whereas those of genes involved in protein biosynthesis are significantly decreased compared with summer active animals in August. Surprisingly, the differentially expressed genes also include adipocyte differentiation-related protein or adipophilin (Adfp), gap junction protein 1 (Gja1), and secreted protein acidic and cysteine-rich (Sparc), which may play a role in enhancing thermogenesis at low tissue temperatures in BAT.

Original languageEnglish (US)
Pages (from-to)346-353
Number of pages8
JournalPhysiological Genomics
Volume25
Issue number2
DOIs
StatePublished - Apr 13 2006
Externally publishedYes

Fingerprint

Hibernation
Sciuridae
Brown Adipose Tissue
Thermogenesis
Gene Expression
Torpor
Genes
Rewarming
Connexins
Temperature
Protein Biosynthesis
Arousal
Body Temperature
Adipocytes
Cysteine
Real-Time Polymerase Chain Reaction
Mammals
Proteins
Messenger RNA

Keywords

  • Metabolism
  • Protein biosynthesis
  • Thermogenesis

ASJC Scopus subject areas

  • Physiology
  • Genetics

Cite this

Detection of differential gene expression in brown adipose tissue of hibernating arctic ground squirrels with mouse microarrays. / Yan, Jun; Burman, Adlai; Nichols, Calen; Alila, Linda; Showe, Louise C.; Showe, Michael K.; Boyer, Bert; Barnes, Brian M.; Marr, Thomas G.

In: Physiological Genomics, Vol. 25, No. 2, 13.04.2006, p. 346-353.

Research output: Contribution to journalArticle

Yan, Jun ; Burman, Adlai ; Nichols, Calen ; Alila, Linda ; Showe, Louise C. ; Showe, Michael K. ; Boyer, Bert ; Barnes, Brian M. ; Marr, Thomas G. / Detection of differential gene expression in brown adipose tissue of hibernating arctic ground squirrels with mouse microarrays. In: Physiological Genomics. 2006 ; Vol. 25, No. 2. pp. 346-353.
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