Detection of cytosine arabinoside resistant cells at low frequency using the bromodeoxyuridine/DNA assay

Frederic M. Waldman; Frank Dolbeare; Joe W. Gray

doi:10.1002/cyto.990060623

Detection of cytosine arabinoside resistant cells at low frequency using the bromodeoxyuridine/DNA assay

Frederic M. Waldman, Frank Dolbeare, Joe W. Gray

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

This report describes a rapid and sensitive procedure for detection of cytosine arabinoside‐ (Ara‐C) resistant mouse leukemia cells (L1210) in a predominantly Ara‐C‐sensitive population. L1210 cell lines sensitive or resistant to Ara‐C were grown and treated with Ara‐C in vitro or in vivo. Ara‐C‐resistant cells were detected as those cells with S‐phase DNA content retaining the ability to incorporate bromodeoxyuridine (BrdUrd) after treatment with Ara‐C. The BrdUrd incorporation ability of the S‐phase cells was assessed by simultaneous flow cytometric measurement of cellular DNA content and amount of incorporated BrdUrd. The proportion of Ara‐C‐resistant cells was accurately estimated at frequencies approaching 10⁻³.

Original language	English (US)
Pages (from-to)	657-662
Number of pages	6
Journal	Cytometry
Volume	6
Issue number	6
DOIs	https://doi.org/10.1002/cyto.990060623
State	Published - Nov 1985
Externally published	Yes

Keywords

Bromodeoxyuridine
cytosine arabinoside
drug resistance
flow cytometry

ASJC Scopus subject areas

Pathology and Forensic Medicine
Biophysics
Hematology
Endocrinology
Cell Biology

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10.1002/cyto.990060623

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title = "Detection of cytosine arabinoside resistant cells at low frequency using the bromodeoxyuridine/DNA assay",

abstract = "This report describes a rapid and sensitive procedure for detection of cytosine arabinoside‐ (Ara‐C) resistant mouse leukemia cells (L1210) in a predominantly Ara‐C‐sensitive population. L1210 cell lines sensitive or resistant to Ara‐C were grown and treated with Ara‐C in vitro or in vivo. Ara‐C‐resistant cells were detected as those cells with S‐phase DNA content retaining the ability to incorporate bromodeoxyuridine (BrdUrd) after treatment with Ara‐C. The BrdUrd incorporation ability of the S‐phase cells was assessed by simultaneous flow cytometric measurement of cellular DNA content and amount of incorporated BrdUrd. The proportion of Ara‐C‐resistant cells was accurately estimated at frequencies approaching 10−3.",

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year = "1985",

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doi = "10.1002/cyto.990060623",

language = "English (US)",

volume = "6",

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T1 - Detection of cytosine arabinoside resistant cells at low frequency using the bromodeoxyuridine/DNA assay

AU - Waldman, Frederic M.

AU - Dolbeare, Frank

AU - Gray, Joe W.

PY - 1985/11

Y1 - 1985/11

N2 - This report describes a rapid and sensitive procedure for detection of cytosine arabinoside‐ (Ara‐C) resistant mouse leukemia cells (L1210) in a predominantly Ara‐C‐sensitive population. L1210 cell lines sensitive or resistant to Ara‐C were grown and treated with Ara‐C in vitro or in vivo. Ara‐C‐resistant cells were detected as those cells with S‐phase DNA content retaining the ability to incorporate bromodeoxyuridine (BrdUrd) after treatment with Ara‐C. The BrdUrd incorporation ability of the S‐phase cells was assessed by simultaneous flow cytometric measurement of cellular DNA content and amount of incorporated BrdUrd. The proportion of Ara‐C‐resistant cells was accurately estimated at frequencies approaching 10−3.

AB - This report describes a rapid and sensitive procedure for detection of cytosine arabinoside‐ (Ara‐C) resistant mouse leukemia cells (L1210) in a predominantly Ara‐C‐sensitive population. L1210 cell lines sensitive or resistant to Ara‐C were grown and treated with Ara‐C in vitro or in vivo. Ara‐C‐resistant cells were detected as those cells with S‐phase DNA content retaining the ability to incorporate bromodeoxyuridine (BrdUrd) after treatment with Ara‐C. The BrdUrd incorporation ability of the S‐phase cells was assessed by simultaneous flow cytometric measurement of cellular DNA content and amount of incorporated BrdUrd. The proportion of Ara‐C‐resistant cells was accurately estimated at frequencies approaching 10−3.

KW - Bromodeoxyuridine

KW - cytosine arabinoside

KW - drug resistance

KW - flow cytometry

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JO - Cytometry

JF - Cytometry

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ER -

Detection of cytosine arabinoside resistant cells at low frequency using the bromodeoxyuridine/DNA assay

Abstract

Keywords

ASJC Scopus subject areas

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