Detection of cytosine arabinoside resistant cells at low frequency using the bromodeoxyuridine/DNA assay

Frederic M. Waldman, Frank Dolbeare, Joe W. Gray

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

This report describes a rapid and sensitive procedure for detection of cytosine arabinoside‐ (Ara‐C) resistant mouse leukemia cells (L1210) in a predominantly Ara‐C‐sensitive population. L1210 cell lines sensitive or resistant to Ara‐C were grown and treated with Ara‐C in vitro or in vivo. Ara‐C‐resistant cells were detected as those cells with S‐phase DNA content retaining the ability to incorporate bromodeoxyuridine (BrdUrd) after treatment with Ara‐C. The BrdUrd incorporation ability of the S‐phase cells was assessed by simultaneous flow cytometric measurement of cellular DNA content and amount of incorporated BrdUrd. The proportion of Ara‐C‐resistant cells was accurately estimated at frequencies approaching 10−3.

Original languageEnglish (US)
Pages (from-to)657-662
Number of pages6
JournalCytometry
Volume6
Issue number6
DOIs
StatePublished - Nov 1985

Keywords

  • Bromodeoxyuridine
  • cytosine arabinoside
  • drug resistance
  • flow cytometry

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biophysics
  • Hematology
  • Endocrinology
  • Cell Biology

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