Detection of cytosine arabinoside resistant cells at low frequency using the bromodeoxyuridine/DNA assay

F. M. Waldman, F. Dolbeare, Joe Gray

Research output: Chapter in Book/Report/Conference proceedingChapter

16 Citations (Scopus)

Abstract

This report describes a rapid and sensitive procedure for detection of cytosine arabinoside- (Ara-C) resistant mouse leukemia cells (L1210) in a predominantly Ara-C sensitive population. L1210 cell lines sensitive or resistant to Ara-C were grown and treated with Ara-C in vitro or in vivo. Ara-C resistant cells were detected as those cells with S-phase DNA content retaining the ability to incorporate bromodeoxyuridine (BrdUrd) after treatment with Ara-C. The BrdUrd incorporation ability of the S-phase cells was assessed by simultaneous flow cytometric measurement of cellular DNA content and amount of incorporated BrdUrd. The proportion of Ara-C-reistant cells was accurately estimated at frequencies approaching 10-3.

Original languageEnglish (US)
Title of host publicationCytometry
Pages657-662
Number of pages6
Volume6
Edition6
StatePublished - 1985
Externally publishedYes

Fingerprint

Cytarabine
Bromodeoxyuridine
DNA
S Phase
Leukemia L1210
Vulnerable Populations
Cell Line

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Waldman, F. M., Dolbeare, F., & Gray, J. (1985). Detection of cytosine arabinoside resistant cells at low frequency using the bromodeoxyuridine/DNA assay. In Cytometry (6 ed., Vol. 6, pp. 657-662)

Detection of cytosine arabinoside resistant cells at low frequency using the bromodeoxyuridine/DNA assay. / Waldman, F. M.; Dolbeare, F.; Gray, Joe.

Cytometry. Vol. 6 6. ed. 1985. p. 657-662.

Research output: Chapter in Book/Report/Conference proceedingChapter

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