Detection of cytomegalovirus in cerebrospinal fluid autopsy specimens from AIDS patients

Cristian L. Achim, Rashed M. Nagra, Rebecca Wang, Jay Nelson, Clayton A. Wiley

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Cytomegalovirus (CMV) is a common opportunistic pathogen infecting AIDS patients. Polymerase chain reaction (PCR) and antigen capture ELISA were used to detect CMV in 40 cerebrospinal fluid autopsy specimens from patients with AIDS. CMV DNA was detected by PCR in 70% of samples. Of the 21 samples from patients with systemic CMV infection, 57% had CMV encephalitis, while 81% had virus in cerebrospinal fluid detectable by PCR. Of the 24 samples from patients with no histologic evidence of CMV encephalitis, 58% had CMV DNA in cerebrospinal fluid detected by PCR. These results suggest that PCR of cerebrospinal fluid sensitively detects systemic CMV infection but is not specific for brain infection in autopsy specimens of AIDS patients.

Original languageEnglish (US)
Pages (from-to)623-627
Number of pages5
JournalJournal of Infectious Diseases
Volume169
Issue number3
StatePublished - Mar 1994
Externally publishedYes

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Cytomegalovirus
Cerebrospinal Fluid
Autopsy
Acquired Immunodeficiency Syndrome
Polymerase Chain Reaction
Cytomegalovirus Infections
Encephalitis
DNA
Enzyme-Linked Immunosorbent Assay
Viruses
Antigens
Brain
Infection

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Detection of cytomegalovirus in cerebrospinal fluid autopsy specimens from AIDS patients. / Achim, Cristian L.; Nagra, Rashed M.; Wang, Rebecca; Nelson, Jay; Wiley, Clayton A.

In: Journal of Infectious Diseases, Vol. 169, No. 3, 03.1994, p. 623-627.

Research output: Contribution to journalArticle

Achim, Cristian L. ; Nagra, Rashed M. ; Wang, Rebecca ; Nelson, Jay ; Wiley, Clayton A. / Detection of cytomegalovirus in cerebrospinal fluid autopsy specimens from AIDS patients. In: Journal of Infectious Diseases. 1994 ; Vol. 169, No. 3. pp. 623-627.
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