Detection of cancer related DNA nanoparticulate biomarkers and nanoparticles in whole blood

Michael J. Heller, Raj Krishnan, Avery Sonnenberg

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

The ability to rapidly isolate and detect DNA nanoparticulate biomarkers (cell free circulating DNA) and drug delivery nanoparticles directly in blood is a major challenge for early disease detection and nanomedicine. We now show that a microarray dielectrophoretic (DEP) device can be used to rapidly isolate and detect high molecular weight (hmw) DNA nanoparticulates and nanoparticles directly from whole blood. At DEP frequencies of 5kHz-10kHz both fluorescent-stained hmw-DNA and 40nm fluorescent nanoparticles separate from the blood and become highly concentrated at specific DEP high field regions over the microelectrodes, while blood cells move to the DEP low field regions. The blood cells can then be removed by a simple fluidic wash while the hmw-DNA and nanoparticles remain highly concentrated. The hmw-DNA could be detected at a level of <260ng/ml, and the nanoparticles at <9.5 × 109 particles/ml, detection levels that are well within the range for viable clinical diagnostics and drug nanoparticle monitoring. DNA stained materials could also be detected directly in blood from patients with Chronic Lymphocytic Leukemia (CLL).

Original languageEnglish (US)
Title of host publicationNanotechnology 2010
Subtitle of host publicationBio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
Pages372-375
Number of pages4
StatePublished - Nov 9 2010
EventNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 - Anaheim, CA, United States
Duration: Jun 21 2010Jun 24 2010

Publication series

NameNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
Volume3

Conference

ConferenceNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
CountryUnited States
CityAnaheim, CA
Period6/21/106/24/10

Keywords

  • Biomarkers
  • Blood
  • Cancer diagnostics
  • Cfc-DNA
  • DNA
  • Dielectrophoresis (DEP)
  • Nanoparticles

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering
  • Electrical and Electronic Engineering

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  • Cite this

    Heller, M. J., Krishnan, R., & Sonnenberg, A. (2010). Detection of cancer related DNA nanoparticulate biomarkers and nanoparticles in whole blood. In Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 (pp. 372-375). (Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010; Vol. 3).