@inproceedings{6d980aed170b4b1887fbdb88ea577837,
title = "Detection of cancer related DNA nanoparticulate biomarkers and nanoparticles in whole blood",
abstract = "The ability to rapidly isolate and detect DNA nanoparticulate biomarkers (cell free circulating DNA) and drug delivery nanoparticles directly in blood is a major challenge for early disease detection and nanomedicine. We now show that a microarray dielectrophoretic (DEP) device can be used to rapidly isolate and detect high molecular weight (hmw) DNA nanoparticulates and nanoparticles directly from whole blood. At DEP frequencies of 5kHz-10kHz both fluorescent-stained hmw-DNA and 40nm fluorescent nanoparticles separate from the blood and become highly concentrated at specific DEP high field regions over the microelectrodes, while blood cells move to the DEP low field regions. The blood cells can then be removed by a simple fluidic wash while the hmw-DNA and nanoparticles remain highly concentrated. The hmw-DNA could be detected at a level of <260ng/ml, and the nanoparticles at <9.5 × 109 particles/ml, detection levels that are well within the range for viable clinical diagnostics and drug nanoparticle monitoring. DNA stained materials could also be detected directly in blood from patients with Chronic Lymphocytic Leukemia (CLL).",
keywords = "Biomarkers, Blood, Cancer diagnostics, Cfc-DNA, DNA, Dielectrophoresis (DEP), Nanoparticles",
author = "Heller, {Michael J.} and Raj Krishnan and Avery Sonnenberg",
year = "2010",
language = "English (US)",
isbn = "9781439834152",
series = "Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010",
pages = "372--375",
booktitle = "Nanotechnology 2010",
note = "Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 ; Conference date: 21-06-2010 Through 24-06-2010",
}