Detection of bcr-abl fusion in chronic myelogeneous leukemia by in situ hybridization

D. C. Tkachuk, C. A. Westbrook, M. Andreeff, T. A. Donlon, M. L. Cleary, K. Suryanarayan, M. Homge, A. Redner, Joe Gray, D. Pinkel

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Abstract

Chronic myelogeneous leukemia (CML) is genetically characterized by fusion of the bcr and abl genes on chromosomes 22 and 9, respectively. In most cases, the fusion involves a reciprocal translocation t(9;22)(q34;q11), which produces the cytogenetically distinctive Philadelphia chromosome (Ph1). Fusion can be detected by Southern (DNA) analysis or by in vitro amplification of the messenger RNA from the fusion gene with polymerase chain reaction (PCR). These techniques are sensitive but cannot be applied to single cells. Two-color fluorescence in situ hybridization (FISH) was used with probes from portions of the bcr and abl genes to detect the bcr-abl fusion in individual blood and bone marrow cells from six patients. The fusion event was detected in all samples analyzed, of which three were cytogenetically Ph1-negative. One of the Ph1-negative samples was also PCR-negative. This approach is fast and sensitive, and provides potential for determining the frequency of the abnormality in different cell lineages.

Original languageEnglish (US)
Pages (from-to)559-562
Number of pages4
JournalScience
Volume250
Issue number4980
StatePublished - Oct 26 1990
Externally publishedYes

Fingerprint

abl Genes
In Situ Hybridization
Leukemia
Chromosomes, Human, Pair 22
Philadelphia Chromosome
Polymerase Chain Reaction
Chromosomes, Human, Pair 9
Gene Fusion
Cell Lineage
Fluorescence In Situ Hybridization
Bone Marrow Cells
Color
Messenger RNA
DNA

ASJC Scopus subject areas

  • General

Cite this

Tkachuk, D. C., Westbrook, C. A., Andreeff, M., Donlon, T. A., Cleary, M. L., Suryanarayan, K., ... Pinkel, D. (1990). Detection of bcr-abl fusion in chronic myelogeneous leukemia by in situ hybridization. Science, 250(4980), 559-562.

Detection of bcr-abl fusion in chronic myelogeneous leukemia by in situ hybridization. / Tkachuk, D. C.; Westbrook, C. A.; Andreeff, M.; Donlon, T. A.; Cleary, M. L.; Suryanarayan, K.; Homge, M.; Redner, A.; Gray, Joe; Pinkel, D.

In: Science, Vol. 250, No. 4980, 26.10.1990, p. 559-562.

Research output: Contribution to journalArticle

Tkachuk, DC, Westbrook, CA, Andreeff, M, Donlon, TA, Cleary, ML, Suryanarayan, K, Homge, M, Redner, A, Gray, J & Pinkel, D 1990, 'Detection of bcr-abl fusion in chronic myelogeneous leukemia by in situ hybridization', Science, vol. 250, no. 4980, pp. 559-562.
Tkachuk DC, Westbrook CA, Andreeff M, Donlon TA, Cleary ML, Suryanarayan K et al. Detection of bcr-abl fusion in chronic myelogeneous leukemia by in situ hybridization. Science. 1990 Oct 26;250(4980):559-562.
Tkachuk, D. C. ; Westbrook, C. A. ; Andreeff, M. ; Donlon, T. A. ; Cleary, M. L. ; Suryanarayan, K. ; Homge, M. ; Redner, A. ; Gray, Joe ; Pinkel, D. / Detection of bcr-abl fusion in chronic myelogeneous leukemia by in situ hybridization. In: Science. 1990 ; Vol. 250, No. 4980. pp. 559-562.
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AU - Tkachuk, D. C.

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AU - Andreeff, M.

AU - Donlon, T. A.

AU - Cleary, M. L.

AU - Suryanarayan, K.

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AU - Redner, A.

AU - Gray, Joe

AU - Pinkel, D.

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N2 - Chronic myelogeneous leukemia (CML) is genetically characterized by fusion of the bcr and abl genes on chromosomes 22 and 9, respectively. In most cases, the fusion involves a reciprocal translocation t(9;22)(q34;q11), which produces the cytogenetically distinctive Philadelphia chromosome (Ph1). Fusion can be detected by Southern (DNA) analysis or by in vitro amplification of the messenger RNA from the fusion gene with polymerase chain reaction (PCR). These techniques are sensitive but cannot be applied to single cells. Two-color fluorescence in situ hybridization (FISH) was used with probes from portions of the bcr and abl genes to detect the bcr-abl fusion in individual blood and bone marrow cells from six patients. The fusion event was detected in all samples analyzed, of which three were cytogenetically Ph1-negative. One of the Ph1-negative samples was also PCR-negative. This approach is fast and sensitive, and provides potential for determining the frequency of the abnormality in different cell lineages.

AB - Chronic myelogeneous leukemia (CML) is genetically characterized by fusion of the bcr and abl genes on chromosomes 22 and 9, respectively. In most cases, the fusion involves a reciprocal translocation t(9;22)(q34;q11), which produces the cytogenetically distinctive Philadelphia chromosome (Ph1). Fusion can be detected by Southern (DNA) analysis or by in vitro amplification of the messenger RNA from the fusion gene with polymerase chain reaction (PCR). These techniques are sensitive but cannot be applied to single cells. Two-color fluorescence in situ hybridization (FISH) was used with probes from portions of the bcr and abl genes to detect the bcr-abl fusion in individual blood and bone marrow cells from six patients. The fusion event was detected in all samples analyzed, of which three were cytogenetically Ph1-negative. One of the Ph1-negative samples was also PCR-negative. This approach is fast and sensitive, and provides potential for determining the frequency of the abnormality in different cell lineages.

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