Detection of bcl-2/JH translocation by polymerase chain reaction: A summary of the experience of the Molecular Oncology Survey of the College of American Pathologists

Eric D. Hsi, Raymond R. Tubbs, Mark A. Lovell, Rita Braziel, Margaret L. Gulley

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Context. - The t(14;18)(q32;q21) translocation, found in about 85% of follicular lymphomas, brings the bcl-2 gene on 18q21 under control of the immunoglobulin heavy-chain gene transcriptional regulatory elements on 14q32. Detection of this translocation in a clinical sample suspected of containing lymphoma can assist the pathologist in diagnosis and classification of lymphoma. Polymerase chain reaction is a technology that is frequently used to detect the t(14;18)(q32;q21) translocation (bcl-2/JH). This article reviews the utility of polymerase chain reaction testing for bcl-2/JH detection and summarizes the experience of participants in the Molecular Oncology Proficiency Survey of the College of American Pathologists from 1997 through 2000. Objective. - To describe current practice and encourage improvement of bcl-2/JH testing in clinical laboratories. Design. - Retrospective analysis of Molecular Oncology Proficiency Survey data. Participants. - Laboratory participants in the College of American Pathology Molecular Oncology Proficiency Survey. Results. - Twenty-four well-characterized specimens were sent to participants, of which 6 contained bcl-2/JH major breakpoint region translocations. Eight hundred nineteen major breakpoint region and 323 minor cluster region determinations were performed, with an overall correct response rate of 91% and 94%, respectively. No significant difference in correct response could be found for frozen versus paraffin-embedded tissues. Many laboratories did not know their assay sensitivity. Conclusions. - Overall performance was good; however, there was great variability in the methods reported and lack of knowledge of the limits of detection was common. Continued participation in external quality control programs, such as the Molecular Oncology Survey; dissemination of information that impacts on test performance; and technical recommendations from the molecular diagnostics community are critical for improved testing for bcl-2/JH.

Original languageEnglish (US)
Pages (from-to)902-908
Number of pages7
JournalArchives of Pathology and Laboratory Medicine
Volume126
Issue number8
StatePublished - Aug 2002
Externally publishedYes

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Polymerase Chain Reaction
Molecular Pathology
Lymphoma
Transcriptional Regulatory Elements
Immunoglobulin Heavy Chain Genes
bcl-2 Genes
Follicular Lymphoma
Information Dissemination
Quality Control
Paraffin
Limit of Detection
Technology
Surveys and Questionnaires
Pathologists

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

Cite this

Detection of bcl-2/JH translocation by polymerase chain reaction : A summary of the experience of the Molecular Oncology Survey of the College of American Pathologists. / Hsi, Eric D.; Tubbs, Raymond R.; Lovell, Mark A.; Braziel, Rita; Gulley, Margaret L.

In: Archives of Pathology and Laboratory Medicine, Vol. 126, No. 8, 08.2002, p. 902-908.

Research output: Contribution to journalArticle

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abstract = "Context. - The t(14;18)(q32;q21) translocation, found in about 85{\%} of follicular lymphomas, brings the bcl-2 gene on 18q21 under control of the immunoglobulin heavy-chain gene transcriptional regulatory elements on 14q32. Detection of this translocation in a clinical sample suspected of containing lymphoma can assist the pathologist in diagnosis and classification of lymphoma. Polymerase chain reaction is a technology that is frequently used to detect the t(14;18)(q32;q21) translocation (bcl-2/JH). This article reviews the utility of polymerase chain reaction testing for bcl-2/JH detection and summarizes the experience of participants in the Molecular Oncology Proficiency Survey of the College of American Pathologists from 1997 through 2000. Objective. - To describe current practice and encourage improvement of bcl-2/JH testing in clinical laboratories. Design. - Retrospective analysis of Molecular Oncology Proficiency Survey data. Participants. - Laboratory participants in the College of American Pathology Molecular Oncology Proficiency Survey. Results. - Twenty-four well-characterized specimens were sent to participants, of which 6 contained bcl-2/JH major breakpoint region translocations. Eight hundred nineteen major breakpoint region and 323 minor cluster region determinations were performed, with an overall correct response rate of 91{\%} and 94{\%}, respectively. No significant difference in correct response could be found for frozen versus paraffin-embedded tissues. Many laboratories did not know their assay sensitivity. Conclusions. - Overall performance was good; however, there was great variability in the methods reported and lack of knowledge of the limits of detection was common. Continued participation in external quality control programs, such as the Molecular Oncology Survey; dissemination of information that impacts on test performance; and technical recommendations from the molecular diagnostics community are critical for improved testing for bcl-2/JH.",
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