Detection of antigen in bronchial epithelium and macrophages in acute Kawasaki disease by use of synthetic antibody

Anne H. Rowley, Susan C. Baker, Stanford T. Shulman, Francesca L. Garcia, Judith Guzman-Cottrill, Pauline Chou, Masaru Terai, Tomisaku Kawasaki, Mitra B. Kalelkar, Susan E. Crawford

Research output: Contribution to journalArticle

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Abstract

Background. Kawasaki disease (KD) is the most common acquired cardiac disease in children in developed nations. The etiology is unknown, but a ubiquitous infectious agent appears to be likely. Immunoglobulin A plasma cells infiltrate inflamed tissues in acute KD, producing oligoclonal, antigen-driven antibodies. Methods. To identify antigens important in the pathogenesis of KD, oligoclonal KD antibodies were prepared in vitro and tested by immunohistochemistry experiments on tissues from patients with acute KD and from control subjects and were also tested for reactivity with human inflammatory proteins. Results. By use of synthetic antibody A, specific binding to a cytoplasmic antigen in proximal bronchial epithelium was observed in 10 of 13 patients with acute KD but in 0 of 9 control subjects (P = .001). A subset of macrophages was positive in at least 1 inflamed tissue from all 17 patients with acute KD. Antigen was detected in 9 of 12 acute KD coronary artery aneurysms but in 0 of 10 control coronary arteries (P

Original languageEnglish (US)
Pages (from-to)856-865
Number of pages10
JournalJournal of Infectious Diseases
Volume190
Issue number4
DOIs
StatePublished - Aug 15 2004
Externally publishedYes

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Mucocutaneous Lymph Node Syndrome
Acute Disease
Epithelium
Macrophages
Antigens
Antibodies
Coronary Vessels
Coronary Aneurysm
Plasma Cells
Developed Countries
Immunoglobulin A
Heart Diseases
Immunohistochemistry

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Detection of antigen in bronchial epithelium and macrophages in acute Kawasaki disease by use of synthetic antibody. / Rowley, Anne H.; Baker, Susan C.; Shulman, Stanford T.; Garcia, Francesca L.; Guzman-Cottrill, Judith; Chou, Pauline; Terai, Masaru; Kawasaki, Tomisaku; Kalelkar, Mitra B.; Crawford, Susan E.

In: Journal of Infectious Diseases, Vol. 190, No. 4, 15.08.2004, p. 856-865.

Research output: Contribution to journalArticle

Rowley, AH, Baker, SC, Shulman, ST, Garcia, FL, Guzman-Cottrill, J, Chou, P, Terai, M, Kawasaki, T, Kalelkar, MB & Crawford, SE 2004, 'Detection of antigen in bronchial epithelium and macrophages in acute Kawasaki disease by use of synthetic antibody', Journal of Infectious Diseases, vol. 190, no. 4, pp. 856-865. https://doi.org/10.1086/422648
Rowley, Anne H. ; Baker, Susan C. ; Shulman, Stanford T. ; Garcia, Francesca L. ; Guzman-Cottrill, Judith ; Chou, Pauline ; Terai, Masaru ; Kawasaki, Tomisaku ; Kalelkar, Mitra B. ; Crawford, Susan E. / Detection of antigen in bronchial epithelium and macrophages in acute Kawasaki disease by use of synthetic antibody. In: Journal of Infectious Diseases. 2004 ; Vol. 190, No. 4. pp. 856-865.
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AU - Rowley, Anne H.

AU - Baker, Susan C.

AU - Shulman, Stanford T.

AU - Garcia, Francesca L.

AU - Guzman-Cottrill, Judith

AU - Chou, Pauline

AU - Terai, Masaru

AU - Kawasaki, Tomisaku

AU - Kalelkar, Mitra B.

AU - Crawford, Susan E.

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N2 - Background. Kawasaki disease (KD) is the most common acquired cardiac disease in children in developed nations. The etiology is unknown, but a ubiquitous infectious agent appears to be likely. Immunoglobulin A plasma cells infiltrate inflamed tissues in acute KD, producing oligoclonal, antigen-driven antibodies. Methods. To identify antigens important in the pathogenesis of KD, oligoclonal KD antibodies were prepared in vitro and tested by immunohistochemistry experiments on tissues from patients with acute KD and from control subjects and were also tested for reactivity with human inflammatory proteins. Results. By use of synthetic antibody A, specific binding to a cytoplasmic antigen in proximal bronchial epithelium was observed in 10 of 13 patients with acute KD but in 0 of 9 control subjects (P = .001). A subset of macrophages was positive in at least 1 inflamed tissue from all 17 patients with acute KD. Antigen was detected in 9 of 12 acute KD coronary artery aneurysms but in 0 of 10 control coronary arteries (P

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