A human chromosomal translocation t(8;9) was detected using two‐color fluorescence in situ hybridization with probes capable of staining the entire lengths of each of these chromosomes. The chromosome 8 probe was labeled with biotin and detected with Texas red, while the chromosome 9 probe was labeled with AAF and detected with FITC. In normal metaphase spreads, two metaphases from the proband, two red, one green and one part red and part green derivative chromosome were seen. The bicolor chromosome corresponded to translocation of a chromosome 8 segment to the distal part of the q region of one chromosome 9, as originally indicated by banding analysis. In interphase nuclei of the proband, four domains with bright fluorescence were recognized in many nuclei. Two were red, one was green, and the fourth had portions of both colors, indicating the presence of the translocation.
|Original language||English (US)|
|Number of pages||6|
|State||Published - Feb 1994|
- DNA probes
- fluorescence in situ hybridization (FISH)
ASJC Scopus subject areas
- Pathology and Forensic Medicine