TY - JOUR
T1 - Detection of a human chromosomal translocation t(8;9) in a baby with multiple malformations using two‐color fluorescence in situ hybridization
AU - Sasaki, Kohsuke
AU - Pinkel, Daniel
AU - Tsukahara, Masato
AU - Murano, Ichiro
AU - Gray, Joe W.
PY - 1994/2
Y1 - 1994/2
N2 - A human chromosomal translocation t(8;9) was detected using two‐color fluorescence in situ hybridization with probes capable of staining the entire lengths of each of these chromosomes. The chromosome 8 probe was labeled with biotin and detected with Texas red, while the chromosome 9 probe was labeled with AAF and detected with FITC. In normal metaphase spreads, two metaphases from the proband, two red, one green and one part red and part green derivative chromosome were seen. The bicolor chromosome corresponded to translocation of a chromosome 8 segment to the distal part of the q region of one chromosome 9, as originally indicated by banding analysis. In interphase nuclei of the proband, four domains with bright fluorescence were recognized in many nuclei. Two were red, one was green, and the fourth had portions of both colors, indicating the presence of the translocation.
AB - A human chromosomal translocation t(8;9) was detected using two‐color fluorescence in situ hybridization with probes capable of staining the entire lengths of each of these chromosomes. The chromosome 8 probe was labeled with biotin and detected with Texas red, while the chromosome 9 probe was labeled with AAF and detected with FITC. In normal metaphase spreads, two metaphases from the proband, two red, one green and one part red and part green derivative chromosome were seen. The bicolor chromosome corresponded to translocation of a chromosome 8 segment to the distal part of the q region of one chromosome 9, as originally indicated by banding analysis. In interphase nuclei of the proband, four domains with bright fluorescence were recognized in many nuclei. Two were red, one was green, and the fourth had portions of both colors, indicating the presence of the translocation.
KW - DNA probes
KW - chromosome
KW - fluorescence in situ hybridization (FISH)
KW - translocation
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U2 - 10.1111/j.1440-1827.1994.tb01699.x
DO - 10.1111/j.1440-1827.1994.tb01699.x
M3 - Article
C2 - 8025653
AN - SCOPUS:0028346966
SN - 1320-5463
VL - 44
SP - 145
EP - 150
JO - Pathology International
JF - Pathology International
IS - 2
ER -