Detailed characterization of tumor infiltrating lymphocytes in two distinct human solid malignancies show phenotypic similarities

Magdalena Kovacsovics-Bankowski, Lana Chisholm, Jonna Vercellini, Christopher G. Tucker, Ryan Montler, Daniel Haley, Philippa Newell, Jun Ma, Paul Tseng, Ronald Wolf, John Vetto, Chet Hammill, Paul Hansen, Andrew D. Weinberg

    Research output: Contribution to journalArticle

    12 Citations (Scopus)

    Abstract

    Background: We examined the phenotype and function of lymphocytes collected from the peripheral blood (PBL) and tumor (TIL) of patients with two different solid malignancies: colorectal cancer liver metastases (CRLM) and ovarian cancer (OVC).Methods: Tumor and corresponding peripheral blood were collected from 16 CRLM and 22 OVC patients; immediately following resection they were processed and analyzed using a multi-color flow cytometry panel. Cytokine mRNA from purified PBL and TIL CD4+ T cells were also analyzed by qPCR.Results: Overall, we found similar changes in the phenotypic and cytokine profiles when the TIL were compared to PBL from patients with two different malignancies. The percentage of Treg (CD4+/CD25+/FoxP3+) in PBL and TIL was similar: 8.1% versus 10.2%, respectively in CRLM patients. However, the frequency of Treg in primary OVC TIL was higher than PBL: 19.2% versus 4.5% (p + T cells (HLA-DR+/CD38+) compared to PBL (CRLM: 30.2% vs 7.7%, (p = 0.0012), OVC: 57.1% vs 12.0%, (p +, CD39+, CTLA-4+ and Helios+) and activated cytotoxic T cells (CD8+/HLA-DR+/CD38+) when compared to PBL and an increase in IL-6 mRNA from CD4 TIL.

    Original languageEnglish (US)
    Article number38
    JournalJournal for ImmunoTherapy of Cancer
    Volume2
    Issue number1
    DOIs
    StatePublished - Nov 18 2014

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    Tumor-Infiltrating Lymphocytes
    Liver Neoplasms
    Ovarian Neoplasms
    Neoplasms
    Neoplasm Metastasis
    HLA-DR Antigens
    T-Lymphocytes
    Cytokines
    Messenger RNA
    Colorectal Neoplasms
    Interleukin-6
    Flow Cytometry
    Color
    Lymphocytes
    Phenotype

    Keywords

    • Regulatory T cells
    • Tumor infiltrating lymphocytes

    ASJC Scopus subject areas

    • Oncology
    • Immunology and Allergy
    • Cancer Research
    • Molecular Medicine
    • Pharmacology
    • Immunology

    Cite this

    Kovacsovics-Bankowski, M., Chisholm, L., Vercellini, J., Tucker, C. G., Montler, R., Haley, D., ... Weinberg, A. D. (2014). Detailed characterization of tumor infiltrating lymphocytes in two distinct human solid malignancies show phenotypic similarities. Journal for ImmunoTherapy of Cancer, 2(1), [38]. https://doi.org/10.1186/s40425-014-0038-9

    Detailed characterization of tumor infiltrating lymphocytes in two distinct human solid malignancies show phenotypic similarities. / Kovacsovics-Bankowski, Magdalena; Chisholm, Lana; Vercellini, Jonna; Tucker, Christopher G.; Montler, Ryan; Haley, Daniel; Newell, Philippa; Ma, Jun; Tseng, Paul; Wolf, Ronald; Vetto, John; Hammill, Chet; Hansen, Paul; Weinberg, Andrew D.

    In: Journal for ImmunoTherapy of Cancer, Vol. 2, No. 1, 38, 18.11.2014.

    Research output: Contribution to journalArticle

    Kovacsovics-Bankowski, M, Chisholm, L, Vercellini, J, Tucker, CG, Montler, R, Haley, D, Newell, P, Ma, J, Tseng, P, Wolf, R, Vetto, J, Hammill, C, Hansen, P & Weinberg, AD 2014, 'Detailed characterization of tumor infiltrating lymphocytes in two distinct human solid malignancies show phenotypic similarities', Journal for ImmunoTherapy of Cancer, vol. 2, no. 1, 38. https://doi.org/10.1186/s40425-014-0038-9
    Kovacsovics-Bankowski, Magdalena ; Chisholm, Lana ; Vercellini, Jonna ; Tucker, Christopher G. ; Montler, Ryan ; Haley, Daniel ; Newell, Philippa ; Ma, Jun ; Tseng, Paul ; Wolf, Ronald ; Vetto, John ; Hammill, Chet ; Hansen, Paul ; Weinberg, Andrew D. / Detailed characterization of tumor infiltrating lymphocytes in two distinct human solid malignancies show phenotypic similarities. In: Journal for ImmunoTherapy of Cancer. 2014 ; Vol. 2, No. 1.
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    AU - Chisholm, Lana

    AU - Vercellini, Jonna

    AU - Tucker, Christopher G.

    AU - Montler, Ryan

    AU - Haley, Daniel

    AU - Newell, Philippa

    AU - Ma, Jun

    AU - Tseng, Paul

    AU - Wolf, Ronald

    AU - Vetto, John

    AU - Hammill, Chet

    AU - Hansen, Paul

    AU - Weinberg, Andrew D.

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    N2 - Background: We examined the phenotype and function of lymphocytes collected from the peripheral blood (PBL) and tumor (TIL) of patients with two different solid malignancies: colorectal cancer liver metastases (CRLM) and ovarian cancer (OVC).Methods: Tumor and corresponding peripheral blood were collected from 16 CRLM and 22 OVC patients; immediately following resection they were processed and analyzed using a multi-color flow cytometry panel. Cytokine mRNA from purified PBL and TIL CD4+ T cells were also analyzed by qPCR.Results: Overall, we found similar changes in the phenotypic and cytokine profiles when the TIL were compared to PBL from patients with two different malignancies. The percentage of Treg (CD4+/CD25+/FoxP3+) in PBL and TIL was similar: 8.1% versus 10.2%, respectively in CRLM patients. However, the frequency of Treg in primary OVC TIL was higher than PBL: 19.2% versus 4.5% (p + T cells (HLA-DR+/CD38+) compared to PBL (CRLM: 30.2% vs 7.7%, (p = 0.0012), OVC: 57.1% vs 12.0%, (p +, CD39+, CTLA-4+ and Helios+) and activated cytotoxic T cells (CD8+/HLA-DR+/CD38+) when compared to PBL and an increase in IL-6 mRNA from CD4 TIL.

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