Design of a confocal fluorescence spectrometer for transdermal observation of green fluorescent protein expression in cartilage

D. S. Gareau, Hong Ma, W. A. Horton, Steven Jacques

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have developed a noninvasive confocal fluorescence spectrometer to measure in vivo fluorescence spectra in mice. Our motivation is the study of the healing process of wounded cartilage in the murine model. The spectrometer measures the expression of green fluorescent protein (GFP) which is linked to the promoter of type II collagen as a marker for the expression of the chondrocyte phenotype. The confocal system uses an argon ion laser (488 nm) to excite fluorescence in a confocal volume about 200 um below the skin surface in the cartilage of the xyphoid process. In vitro studies showed how the refractive mismatch at the surface boundary and the light scattering of the tissue affect the depth and size of the confocal volume. A thin fluorescent layer was detected through a 130 um tissue phantom (murine skin) by moving the confocal volume axially through the phantom. The presence of skin increased the axial full width at half maximum of the confocal response from 7 um to 72 um. In conclusion, we achieve a low-spatial-resolution confocal spectrometer, which yields a fluorescence spectrum that maximizes the GFP fluorescence of cartilage and minimizes the skin autofluorescence.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsJ. Conchello, C.J. Cogswell, T. Wilson
Pages132-135
Number of pages4
Volume4261
DOIs
StatePublished - 2001
EventThree-Dimensional and Multidimensional Microscopy: Image Asquisition and Processin VIII - San Jose, CA, United States
Duration: Jan 21 2001Jan 22 2001

Other

OtherThree-Dimensional and Multidimensional Microscopy: Image Asquisition and Processin VIII
CountryUnited States
CitySan Jose, CA
Period1/21/011/22/01

Fingerprint

cartilage
Cartilage
Spectrometers
Fluorescence
spectrometers
proteins
Proteins
Skin
fluorescence
Tissue
phenotype
healing
collagens
Full width at half maximum
Collagen
Light scattering
markers
mice
Argon
light scattering

Keywords

  • Axial point spread function
  • Confocal response
  • Confocal volume
  • Murine skin

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Gareau, D. S., Ma, H., Horton, W. A., & Jacques, S. (2001). Design of a confocal fluorescence spectrometer for transdermal observation of green fluorescent protein expression in cartilage. In J. Conchello, C. J. Cogswell, & T. Wilson (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4261, pp. 132-135) https://doi.org/10.1117/12.424528

Design of a confocal fluorescence spectrometer for transdermal observation of green fluorescent protein expression in cartilage. / Gareau, D. S.; Ma, Hong; Horton, W. A.; Jacques, Steven.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / J. Conchello; C.J. Cogswell; T. Wilson. Vol. 4261 2001. p. 132-135.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Gareau, DS, Ma, H, Horton, WA & Jacques, S 2001, Design of a confocal fluorescence spectrometer for transdermal observation of green fluorescent protein expression in cartilage. in J Conchello, CJ Cogswell & T Wilson (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4261, pp. 132-135, Three-Dimensional and Multidimensional Microscopy: Image Asquisition and Processin VIII, San Jose, CA, United States, 1/21/01. https://doi.org/10.1117/12.424528
Gareau DS, Ma H, Horton WA, Jacques S. Design of a confocal fluorescence spectrometer for transdermal observation of green fluorescent protein expression in cartilage. In Conchello J, Cogswell CJ, Wilson T, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 4261. 2001. p. 132-135 https://doi.org/10.1117/12.424528
Gareau, D. S. ; Ma, Hong ; Horton, W. A. ; Jacques, Steven. / Design of a confocal fluorescence spectrometer for transdermal observation of green fluorescent protein expression in cartilage. Proceedings of SPIE - The International Society for Optical Engineering. editor / J. Conchello ; C.J. Cogswell ; T. Wilson. Vol. 4261 2001. pp. 132-135
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