Design and calibration of a digital Fourier holographic microscope for particle sizing via goniometry and optical scatter imaging in transmission

Vincent M. Rossi, Steven Jacques

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Abstract

Goniometry and optical scatter imaging have been used for optical determination of particle size based upon optical scattering. Polystyrene microspheres in suspension serve as a standard for system validation purposes. The design and calibration of a digital Fourier holographic microscope (DFHM) are reported. Of crucial importance is the appropriate scaling of scattering angle space in the conjugate Fourier plane. A detailed description of this calibration process is described. Spatial filtering of the acquired digital hologram to use photons scattered within a restricted angular range produces an image. A pair of images, one using photons narrowly scattered within 8-15o (LNA), and one using photons broadly scattered within 8-39o (HNA), are produced. An image based on the ratio of these two images, OSIR = HNA=LNA, following Boustany et al. (2002), yields a 2D Optical Scatter Image (OSI) whose contrast is based on the angular dependence of photon scattering and is sensitive to the microsphere size, especially in the 0:5-1:0mm range. Goniometric results are also given for polystyrene microspheres in suspension as additional proof of principle for particle sizing via the DFHM.

Original languageEnglish (US)
Pages (from-to)12788-12802
Number of pages15
JournalOptics Express
Volume24
Issue number12
DOIs
StatePublished - Jun 13 2016

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ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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