Desensitization of AMPA receptors upon multiquantal neurotransmitter release

Laurence Trussell, Su Zhang, Indira M. Ramant

Research output: Contribution to journalArticle

346 Citations (Scopus)

Abstract

We have investigated the role of AMPA receptor desensitization during transmission at a calyceal synapse. Cyclothiazide blocked the rapid desensitization of AMPA receptors and markedly prolonged the decay time of the evoked excitatory postsynaptic current (PSC). This effect was greater than what would be expected from a simple prolongation of channel open time. Additionally, the drug reduced the depression of PSCs evoked at brief intervals. The effects of cyclothiazide on the PSC were reduced when the level of transmitter release was lowered. These data indicate that AMPA receptors are desensitized by neurotransmitter and that this desensitization depends on the number of quanta in the synaptic cleft. We propose that release of transmitter from many closely spaced sites prolongs the time of receptor-transmitter contact and thereby promotes desensitization. Desensitization may therefore contribute to synaptic depression and prevent the interaction of transmitter quanta within the synaptic cleft.

Original languageEnglish (US)
Pages (from-to)1185-1196
Number of pages12
JournalNeuron
Volume10
Issue number6
DOIs
StatePublished - 1993
Externally publishedYes

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AMPA Receptors
Neurotransmitter Agents
Synaptic Potentials
Excitatory Postsynaptic Potentials
Synapses
Pharmaceutical Preparations
cyclothiazide

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Desensitization of AMPA receptors upon multiquantal neurotransmitter release. / Trussell, Laurence; Zhang, Su; Ramant, Indira M.

In: Neuron, Vol. 10, No. 6, 1993, p. 1185-1196.

Research output: Contribution to journalArticle

Trussell, Laurence ; Zhang, Su ; Ramant, Indira M. / Desensitization of AMPA receptors upon multiquantal neurotransmitter release. In: Neuron. 1993 ; Vol. 10, No. 6. pp. 1185-1196.
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