Description of a new hamster ventral prostate cell line containing androgen receptors

James S. Norris, Charles Bowden, Peter Kohler

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Using chemical transformation of primary monolayers of hamster ventral prostate tissue, we have established the HVP-G3 and HVP-B1 cloned cell lines which have been growing in our laboratories for over 18 months. Both cell lines contain high-affinity, saturable androgen receptors which migrate into the nucleus when exposed to physiological doses of androgen. However, neither cell line demonstrates a growth response to androgens. Each cell line will produce a tumor in 6 to 8 weeks when injected subcutaneously into male or female Syrian hamsters. In vitro cell doubling times range from 12 hr in the presence of luteinizing hormone (LH-NIH-S-18) to 18 hr in the presence of 5% serum. Both cell lines have a diploid stemline.

Original languageEnglish (US)
Pages (from-to)108-114
Number of pages7
JournalIn Vitro
Volume13
Issue number2
DOIs
StatePublished - Feb 1977
Externally publishedYes

Fingerprint

Androgen Receptors
Cricetinae
Prostate
Cells
Cell Line
Androgens
Mesocricetus
Luteinizing Hormone
Diploidy
Tumors
Monolayers
Tissue
Growth
Serum
Neoplasms

Keywords

  • androgen receptors
  • cell line
  • chemical transformation
  • prostate

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Description of a new hamster ventral prostate cell line containing androgen receptors. / Norris, James S.; Bowden, Charles; Kohler, Peter.

In: In Vitro, Vol. 13, No. 2, 02.1977, p. 108-114.

Research output: Contribution to journalArticle

Norris, James S. ; Bowden, Charles ; Kohler, Peter. / Description of a new hamster ventral prostate cell line containing androgen receptors. In: In Vitro. 1977 ; Vol. 13, No. 2. pp. 108-114.
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