Description of a new hamster ventral prostate cell line containing androgen receptors

James S. Norris, Charles Bowden, P. O. Kohler

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Using chemical transformation of primary monolayers of hamster ventral prostate tissue, we have established the HVP-G3 and HVP-B1 cloned cell lines which have been growing in our laboratories for over 18 months. Both cell lines contain high-affinity, saturable androgen receptors which migrate into the nucleus when exposed to physiological doses of androgen. However, neither cell line demonstrates a growth response to androgens. Each cell line will produce a tumor in 6 to 8 weeks when injected subcutaneously into male or female Syrian hamsters. In vitro cell doubling times range from 12 hr in the presence of luteinizing hormone (LH-NIH-S-18) to 18 hr in the presence of 5% serum. Both cell lines have a diploid stemline.

Original languageEnglish (US)
Pages (from-to)108-114
Number of pages7
JournalIn Vitro
Volume13
Issue number2
DOIs
StatePublished - Feb 1 1977

Keywords

  • androgen receptors
  • cell line
  • chemical transformation
  • prostate

ASJC Scopus subject areas

  • Biotechnology
  • Plant Science

Fingerprint Dive into the research topics of 'Description of a new hamster ventral prostate cell line containing androgen receptors'. Together they form a unique fingerprint.

  • Cite this