Abstract
Using chemical transformation of primary monolayers of hamster ventral prostate tissue, we have established the HVP-G3 and HVP-B1 cloned cell lines which have been growing in our laboratories for over 18 months. Both cell lines contain high-affinity, saturable androgen receptors which migrate into the nucleus when exposed to physiological doses of androgen. However, neither cell line demonstrates a growth response to androgens. Each cell line will produce a tumor in 6 to 8 weeks when injected subcutaneously into male or female Syrian hamsters. In vitro cell doubling times range from 12 hr in the presence of luteinizing hormone (LH-NIH-S-18) to 18 hr in the presence of 5% serum. Both cell lines have a diploid stemline.
Original language | English (US) |
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Pages (from-to) | 108-114 |
Number of pages | 7 |
Journal | In Vitro |
Volume | 13 |
Issue number | 2 |
DOIs | |
State | Published - Feb 1977 |
Externally published | Yes |
Keywords
- androgen receptors
- cell line
- chemical transformation
- prostate
ASJC Scopus subject areas
- Biotechnology
- Plant Science