TY - JOUR
T1 - Dermal reflectivity determined by optical coherence tomography is an indicator of epidermal hyperplasia and dermal edema within inflamed skin
AU - Phillips, Kevin G.
AU - Wang, Yun
AU - Levitz, David
AU - Choudhury, Niloy
AU - Swanzey, Emily
AU - Lagowski, James
AU - Kulesz-Martin, Molly
AU - Jacques, Steven L.
N1 - Funding Information:
We thank the reviewers for their careful review and constructive criticisms, Dr. Ricky Wang for useful discussions, and Dr. Andrew Blauvelt for a careful read of the manuscript. Sawan Hurst, Trevor Levin, and the McCarty Lab provided technical assistance. Histology was performed by Carolyn Gendron of the Chris Corless Lab. We acknowledge support from National Institutes of Health under Grant Nos. 1U54CA143906-01 (K.P.), R01CA098577 (M.K.M., J.L., E.S.), and R01AR055651 (Y.W., M.K.M., J.L., E.S.), and R01HL084013 (S.L.J.).
PY - 2011/4
Y1 - 2011/4
N2 - Psoriasis is a common inflammatory skin disease resulting from genetic and environmental alterations of cutaneous immune responses. While numerous therapeutic targets involved in the immunopathogenesis of psoriasis have been identified, the in vivo dynamics of inflammation in psoriasis remain unclear. We undertook in vivo time course focus-tracked optical coherence tomography (OCT) imaging to noninvasively document cutaneous alterations in mouse skin treated topically with Imiquimod (IMQ), an established model of a psoriasis-like disease. Quantitative appraisal of dermal architectural changes was achieved through a two parameter fit of OCT axial scans in the dermis of the form A(x, y, z) (x, y)exp [- (x, y)z]. Ensemble averaging over 2000 axial scans per mouse in each treatment arm revealed no significant changes in the average dermal attenuation rate, , however the average local dermal reflectivity , decreased significantly following 1, 3, and 6 days of IMQ treatment (p 0.001) in comparison to vehicle-treated control mice. In contrast, epidermal and dermal thickness changes were only significant when comparing controls and 6-day IMQ treated mice. This suggests that dermal alterations, attributed to collagen fiber bundle enlargement, occur prior to epidermal thickness changes due to hyperplasia and dermal thickness changes due to edema. Dermal reflectivity positively correlated with epidermal hyperplasia (r epi 2 = 0.78) and dermal edema (r derm 2 = 0.86). Our results suggest that dermal reflectivity as measured by OCT can be utilized to quantify a psoriasis-like disease in mice, and thus has the potential to aid in the quantitative assessment of psoriasis in humans.
AB - Psoriasis is a common inflammatory skin disease resulting from genetic and environmental alterations of cutaneous immune responses. While numerous therapeutic targets involved in the immunopathogenesis of psoriasis have been identified, the in vivo dynamics of inflammation in psoriasis remain unclear. We undertook in vivo time course focus-tracked optical coherence tomography (OCT) imaging to noninvasively document cutaneous alterations in mouse skin treated topically with Imiquimod (IMQ), an established model of a psoriasis-like disease. Quantitative appraisal of dermal architectural changes was achieved through a two parameter fit of OCT axial scans in the dermis of the form A(x, y, z) (x, y)exp [- (x, y)z]. Ensemble averaging over 2000 axial scans per mouse in each treatment arm revealed no significant changes in the average dermal attenuation rate, , however the average local dermal reflectivity , decreased significantly following 1, 3, and 6 days of IMQ treatment (p 0.001) in comparison to vehicle-treated control mice. In contrast, epidermal and dermal thickness changes were only significant when comparing controls and 6-day IMQ treated mice. This suggests that dermal alterations, attributed to collagen fiber bundle enlargement, occur prior to epidermal thickness changes due to hyperplasia and dermal thickness changes due to edema. Dermal reflectivity positively correlated with epidermal hyperplasia (r epi 2 = 0.78) and dermal edema (r derm 2 = 0.86). Our results suggest that dermal reflectivity as measured by OCT can be utilized to quantify a psoriasis-like disease in mice, and thus has the potential to aid in the quantitative assessment of psoriasis in humans.
KW - dermatology
KW - edema
KW - optical coherence tomography
KW - psoriasis
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U2 - 10.1117/1.3567082
DO - 10.1117/1.3567082
M3 - Article
C2 - 21529065
AN - SCOPUS:79953795927
SN - 1083-3668
VL - 16
JO - Journal of biomedical optics
JF - Journal of biomedical optics
IS - 4
M1 - 040503
ER -