Denudations as paracellular routes for alphafetoprotein and creatinine across the human syncytiotrophoblast

P. Brownbill, D. Mahendran, D. Owen, P. Swanson, Kent Thornburg, D. M. Nelson, C. P. Sibley

    Research output: Contribution to journalArticle

    42 Citations (Scopus)

    Abstract

    We tested two hypotheses: 1) that fibrin-containing fibrinoid-filled denudations of the syncytiotrophoblast may provide a route for paracellular diffusion and 2) that placentas from women who had elevated maternal serum alphafetoprotein (MSAFP) in midgestation had raised permeability to AFP and greater denudation than in normal pregnancy. We measured AFP and creatinine clearance across term placental cotyledons from the above groups and used light microscope morphometric analysis to determine the volume density of fibrin-containing fibrinoid deposits. There was no significant difference between the two groups in terms of AFP and creatinine clearance or volume density of fibrin-containing fibrinold deposits. The combined data showed a significant (P <0.05) positive correlation between creatinine clearance, but not AFP clearance, and volume density of fibrin-containing fibrinoid. We conclude that syncytiotrophoblast denudations, with associated fibrinoid, do provide a route for diffusion of small hydrophilic solutes, but that other anatomic features of the placenta are rate limiting for transfer of AFP and similarly sized molecules.

    Original languageEnglish (US)
    JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
    Volume278
    Issue number3 47-3
    StatePublished - Mar 2000

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    Trophoblasts
    Fibrin
    Creatinine
    Placenta
    Cotyledon
    Permeability
    Mothers
    Light
    Pregnancy
    Serum

    Keywords

    • Fibrin-containing fibrinoid
    • Maternal serum alphafetoprotein
    • Placenta

    ASJC Scopus subject areas

    • Physiology
    • Physiology (medical)

    Cite this

    Denudations as paracellular routes for alphafetoprotein and creatinine across the human syncytiotrophoblast. / Brownbill, P.; Mahendran, D.; Owen, D.; Swanson, P.; Thornburg, Kent; Nelson, D. M.; Sibley, C. P.

    In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 278, No. 3 47-3, 03.2000.

    Research output: Contribution to journalArticle

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    AU - Mahendran, D.

    AU - Owen, D.

    AU - Swanson, P.

    AU - Thornburg, Kent

    AU - Nelson, D. M.

    AU - Sibley, C. P.

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