Dentin matrix component solubilization by solutions of pH relevant to self-etching dental adhesives.

Jack Ferracane, Paul R. Cooper, Anthony J. Smith

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

To evaluate the potential for phosphoric acid solutions - common constituents of dental adhesive systems - of varying pH to solubilize dentin matrix components (DMCs) from human dentin. Human dentin chips were ground under liquid nitrogen to a powder (ca 100 μm) and incubated at 4°C with agitation in phosphoric acid of pH 1, 2, 3, 4, 5, and 6 (1 g/4 ml; n = 4) for six days with solution changes each day. Estimates of daily protein release were made by UV spectrophotometry at 280 nm. Extract solutions were dialyzed for 7 days in reverse osmosis water, lyophilized, and weighed. Non-collagenous proteins (NCPs) and glycosaminoglycans (GAGs) were quantitated by dye-binding assays. 1D-PAGE for preliminary protein characterization and sandwich ELISA for presence of TGF-ß1 were performed. The results were analyzed by ANOVA and regression (α

Original languageEnglish (US)
Pages (from-to)407-412
Number of pages6
JournalThe journal of adhesive dentistry
Volume15
Issue number5
StatePublished - Oct 2013

Fingerprint

Dental Cements
Dentin
Osmosis
Proteins
Spectrophotometry
Glycosaminoglycans
Powders
Analysis of Variance
Coloring Agents
Nitrogen
Enzyme-Linked Immunosorbent Assay
Water
phosphoric acid

ASJC Scopus subject areas

  • Dentistry(all)
  • Medicine(all)

Cite this

Dentin matrix component solubilization by solutions of pH relevant to self-etching dental adhesives. / Ferracane, Jack; Cooper, Paul R.; Smith, Anthony J.

In: The journal of adhesive dentistry, Vol. 15, No. 5, 10.2013, p. 407-412.

Research output: Contribution to journalArticle

@article{905d2bd798dd45a09c3fa0d9c51785ca,
title = "Dentin matrix component solubilization by solutions of pH relevant to self-etching dental adhesives.",
abstract = "To evaluate the potential for phosphoric acid solutions - common constituents of dental adhesive systems - of varying pH to solubilize dentin matrix components (DMCs) from human dentin. Human dentin chips were ground under liquid nitrogen to a powder (ca 100 μm) and incubated at 4°C with agitation in phosphoric acid of pH 1, 2, 3, 4, 5, and 6 (1 g/4 ml; n = 4) for six days with solution changes each day. Estimates of daily protein release were made by UV spectrophotometry at 280 nm. Extract solutions were dialyzed for 7 days in reverse osmosis water, lyophilized, and weighed. Non-collagenous proteins (NCPs) and glycosaminoglycans (GAGs) were quantitated by dye-binding assays. 1D-PAGE for preliminary protein characterization and sandwich ELISA for presence of TGF-{\ss}1 were performed. The results were analyzed by ANOVA and regression (α",
author = "Jack Ferracane and Cooper, {Paul R.} and Smith, {Anthony J.}",
year = "2013",
month = "10",
language = "English (US)",
volume = "15",
pages = "407--412",
journal = "Journal of Adhesive Dentistry",
issn = "1461-5185",
publisher = "Quintessence Publishing Company",
number = "5",

}

TY - JOUR

T1 - Dentin matrix component solubilization by solutions of pH relevant to self-etching dental adhesives.

AU - Ferracane, Jack

AU - Cooper, Paul R.

AU - Smith, Anthony J.

PY - 2013/10

Y1 - 2013/10

N2 - To evaluate the potential for phosphoric acid solutions - common constituents of dental adhesive systems - of varying pH to solubilize dentin matrix components (DMCs) from human dentin. Human dentin chips were ground under liquid nitrogen to a powder (ca 100 μm) and incubated at 4°C with agitation in phosphoric acid of pH 1, 2, 3, 4, 5, and 6 (1 g/4 ml; n = 4) for six days with solution changes each day. Estimates of daily protein release were made by UV spectrophotometry at 280 nm. Extract solutions were dialyzed for 7 days in reverse osmosis water, lyophilized, and weighed. Non-collagenous proteins (NCPs) and glycosaminoglycans (GAGs) were quantitated by dye-binding assays. 1D-PAGE for preliminary protein characterization and sandwich ELISA for presence of TGF-ß1 were performed. The results were analyzed by ANOVA and regression (α

AB - To evaluate the potential for phosphoric acid solutions - common constituents of dental adhesive systems - of varying pH to solubilize dentin matrix components (DMCs) from human dentin. Human dentin chips were ground under liquid nitrogen to a powder (ca 100 μm) and incubated at 4°C with agitation in phosphoric acid of pH 1, 2, 3, 4, 5, and 6 (1 g/4 ml; n = 4) for six days with solution changes each day. Estimates of daily protein release were made by UV spectrophotometry at 280 nm. Extract solutions were dialyzed for 7 days in reverse osmosis water, lyophilized, and weighed. Non-collagenous proteins (NCPs) and glycosaminoglycans (GAGs) were quantitated by dye-binding assays. 1D-PAGE for preliminary protein characterization and sandwich ELISA for presence of TGF-ß1 were performed. The results were analyzed by ANOVA and regression (α

UR - http://www.scopus.com/inward/record.url?scp=84893035158&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84893035158&partnerID=8YFLogxK

M3 - Article

C2 - 23560260

AN - SCOPUS:84893035158

VL - 15

SP - 407

EP - 412

JO - Journal of Adhesive Dentistry

JF - Journal of Adhesive Dentistry

SN - 1461-5185

IS - 5

ER -