Dentin matrix component solubilization by solutions of pH relevant to self-etching dental adhesives.

Jack Ferracane, Paul R. Cooper, Anthony J. Smith

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Abstract

To evaluate the potential for phosphoric acid solutions - common constituents of dental adhesive systems - of varying pH to solubilize dentin matrix components (DMCs) from human dentin. Human dentin chips were ground under liquid nitrogen to a powder (ca 100 μm) and incubated at 4°C with agitation in phosphoric acid of pH 1, 2, 3, 4, 5, and 6 (1 g/4 ml; n = 4) for six days with solution changes each day. Estimates of daily protein release were made by UV spectrophotometry at 280 nm. Extract solutions were dialyzed for 7 days in reverse osmosis water, lyophilized, and weighed. Non-collagenous proteins (NCPs) and glycosaminoglycans (GAGs) were quantitated by dye-binding assays. 1D-PAGE for preliminary protein characterization and sandwich ELISA for presence of TGF-ß1 were performed. The results were analyzed by ANOVA and regression (α

Original languageEnglish (US)
Pages (from-to)407-412
Number of pages6
JournalThe journal of adhesive dentistry
Volume15
Issue number5
Publication statusPublished - Oct 2013

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ASJC Scopus subject areas

  • Dentistry(all)
  • Medicine(all)

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