Density distribution of myelin fragments isolated from control and multiple sclerosis brains

Gregory Konat, Halina Offner

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Myelin from subcortical normal-appearing white matter of control and multiple sclerosis (MS) brains was isolated and subsequently subfractionated on discontinuous sucrose gradients. Three following myelin subfractions were obtained: light myelin (buoyant density ≤ 0.625 M), medium myelin (0.625 M > buoyant density ≤ 0.7 M), and heavy myelin (buoyant density > 0.7 M). The yield of total myelin (the sum of all three subfractions) recovered from MS specimens was about 30% lower than that from the white matter of the control brains. Furthermore, MS myelin was deficient in the light subfraction and was enriched in the heavy subfraction. No abnormality in lipid composition of MS subfractions was observed. On the other hand, myelin particles isolated from the MS tissue were depleted in basic protein. The results are interpreted as an evidence for a rather diffused pathological process in MS white matter.

Original languageEnglish (US)
Pages (from-to)241-246
Number of pages6
JournalNeurochemistry International
Volume4
Issue number4
DOIs
StatePublished - 1982
Externally publishedYes

Fingerprint

Myelin Sheath
Multiple Sclerosis
Brain
Pathologic Processes
Sucrose
Lipids
Light
White Matter

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Density distribution of myelin fragments isolated from control and multiple sclerosis brains. / Konat, Gregory; Offner, Halina.

In: Neurochemistry International, Vol. 4, No. 4, 1982, p. 241-246.

Research output: Contribution to journalArticle

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abstract = "Myelin from subcortical normal-appearing white matter of control and multiple sclerosis (MS) brains was isolated and subsequently subfractionated on discontinuous sucrose gradients. Three following myelin subfractions were obtained: light myelin (buoyant density ≤ 0.625 M), medium myelin (0.625 M > buoyant density ≤ 0.7 M), and heavy myelin (buoyant density > 0.7 M). The yield of total myelin (the sum of all three subfractions) recovered from MS specimens was about 30{\%} lower than that from the white matter of the control brains. Furthermore, MS myelin was deficient in the light subfraction and was enriched in the heavy subfraction. No abnormality in lipid composition of MS subfractions was observed. On the other hand, myelin particles isolated from the MS tissue were depleted in basic protein. The results are interpreted as an evidence for a rather diffused pathological process in MS white matter.",
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