Demonstration of δRec-pseudo Jα rearrangement with deletion of the δ locus in a human stem-cell leukemia

C. G. Begley, P. D. Aplan, Michael Davey, J. P. De Villartay, D. I. Cohen, T. A. Waldmann, I. R. Kirsch

Research output: Contribution to journalArticle

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Abstract

It has been hypothesized that a rearrangement between the δ recombining element (δRec) and a pseudo Jα gene serves to delete the TCR-δ locus before rearrangement of the TCR-α genes. We have now sequenced a direct, site-specific rearrangement between the δRec element and a pseudo Jα gene in a human leukemic stem-cell line. Putative 'N-sequence' addition was noted at the site of recombination, suggesting that this even occurred at the time when the enzyme(s) involved in N-region addition were active in this cell. This provides support for the view that deletion of the TCR-δ locus is required before rearrangement of the TCR-α chain genes.

Original languageEnglish (US)
Pages (from-to)339-342
Number of pages4
JournalJournal of Experimental Medicine
Volume170
Issue number1
StatePublished - 1989
Externally publishedYes

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Leukemia
Stem Cells
Genes
Gene Rearrangement
Genetic Recombination
Cell Line
Enzymes

ASJC Scopus subject areas

  • Immunology

Cite this

Begley, C. G., Aplan, P. D., Davey, M., De Villartay, J. P., Cohen, D. I., Waldmann, T. A., & Kirsch, I. R. (1989). Demonstration of δRec-pseudo Jα rearrangement with deletion of the δ locus in a human stem-cell leukemia. Journal of Experimental Medicine, 170(1), 339-342.

Demonstration of δRec-pseudo Jα rearrangement with deletion of the δ locus in a human stem-cell leukemia. / Begley, C. G.; Aplan, P. D.; Davey, Michael; De Villartay, J. P.; Cohen, D. I.; Waldmann, T. A.; Kirsch, I. R.

In: Journal of Experimental Medicine, Vol. 170, No. 1, 1989, p. 339-342.

Research output: Contribution to journalArticle

Begley, CG, Aplan, PD, Davey, M, De Villartay, JP, Cohen, DI, Waldmann, TA & Kirsch, IR 1989, 'Demonstration of δRec-pseudo Jα rearrangement with deletion of the δ locus in a human stem-cell leukemia', Journal of Experimental Medicine, vol. 170, no. 1, pp. 339-342.
Begley, C. G. ; Aplan, P. D. ; Davey, Michael ; De Villartay, J. P. ; Cohen, D. I. ; Waldmann, T. A. ; Kirsch, I. R. / Demonstration of δRec-pseudo Jα rearrangement with deletion of the δ locus in a human stem-cell leukemia. In: Journal of Experimental Medicine. 1989 ; Vol. 170, No. 1. pp. 339-342.
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