Delivery of active hexosaminidase across the blood-brain barrier in rats

Edward A. Neuwelt, John A. Barranger, Michael A. Pagel, Jane M. Quirk, Roscoe O. Brady, Eugene P. Frenkel

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The ability to deliver enzymatically active human hexosaminidase A across the blood-brain barrier and into brain cells of the normal rat was examined. Following osmotic blood-brain barrier modification in the rat, infra- arterially administered human hexosaminidase A and B were shown to cross the barrier and enter brain cells. Subcellular fractionation studies demonstrated that most of the human enzyme delivered across the barrier was functionally active and appeared to be inside a subcellular organelle. These studies provide evidence that blood-brain barrier modification permits delivery of functionally active hexosaminidase A into subcellular organelles consistent with that known to be the appropriate site of physiologic activity.

Original languageEnglish (US)
Pages (from-to)1012-1019
Number of pages8
JournalNeurology
Volume34
Issue number8
DOIs
StatePublished - Aug 1984

ASJC Scopus subject areas

  • Clinical Neurology

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    Neuwelt, E. A., Barranger, J. A., Pagel, M. A., Quirk, J. M., Brady, R. O., & Frenkel, E. P. (1984). Delivery of active hexosaminidase across the blood-brain barrier in rats. Neurology, 34(8), 1012-1019. https://doi.org/10.1212/wnl.34.8.1012