Delivery of active hexosaminidase across the blood-brain barrier in rats

Edward Neuwelt, John A. Barranger, Michael A. Pagel, Jane M. Quirk, Roscoe O. Brady, Eugene P. Frenkel

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The ability to deliver enzymatically active human hexosaminidase A across the blood-brain barrier and into brain cells of the normal rat was examined. Following osmotic blood-brain barrier modification in the rat, infra- arterially administered human hexosaminidase A and B were shown to cross the barrier and enter brain cells. Subcellular fractionation studies demonstrated that most of the human enzyme delivered across the barrier was functionally active and appeared to be inside a subcellular organelle. These studies provide evidence that blood-brain barrier modification permits delivery of functionally active hexosaminidase A into subcellular organelles consistent with that known to be the appropriate site of physiologic activity.

Original languageEnglish (US)
Pages (from-to)1012-1019
Number of pages8
JournalNeurology
Volume34
Issue number8
StatePublished - 1984

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Hexosaminidase A
Hexosaminidases
Blood-Brain Barrier
Organelles
Hexosaminidase B
Brain
Enzymes
Rat
Blood

ASJC Scopus subject areas

  • Arts and Humanities (miscellaneous)
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Neuwelt, E., Barranger, J. A., Pagel, M. A., Quirk, J. M., Brady, R. O., & Frenkel, E. P. (1984). Delivery of active hexosaminidase across the blood-brain barrier in rats. Neurology, 34(8), 1012-1019.

Delivery of active hexosaminidase across the blood-brain barrier in rats. / Neuwelt, Edward; Barranger, John A.; Pagel, Michael A.; Quirk, Jane M.; Brady, Roscoe O.; Frenkel, Eugene P.

In: Neurology, Vol. 34, No. 8, 1984, p. 1012-1019.

Research output: Contribution to journalArticle

Neuwelt, E, Barranger, JA, Pagel, MA, Quirk, JM, Brady, RO & Frenkel, EP 1984, 'Delivery of active hexosaminidase across the blood-brain barrier in rats', Neurology, vol. 34, no. 8, pp. 1012-1019.
Neuwelt E, Barranger JA, Pagel MA, Quirk JM, Brady RO, Frenkel EP. Delivery of active hexosaminidase across the blood-brain barrier in rats. Neurology. 1984;34(8):1012-1019.
Neuwelt, Edward ; Barranger, John A. ; Pagel, Michael A. ; Quirk, Jane M. ; Brady, Roscoe O. ; Frenkel, Eugene P. / Delivery of active hexosaminidase across the blood-brain barrier in rats. In: Neurology. 1984 ; Vol. 34, No. 8. pp. 1012-1019.
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