@inproceedings{2391ac6327134a4fa8442b5e27f44c54,
title = "Delivering dye into cultured cells using infrared free-electron laser",
abstract = "Free electron lasers (FELs) can be used to molecular operation such as the delivery of a number of molecules into cells. Cultured NIH3T3 cells are exposed to high-intensity short pulse FEL. The FEL is tuned to an absorption maximum wavelength, 6.1 μm, which was measured by microscopic FTIR. A fluorescence dye in the cell suspension is more absorbed into the cell with the FEL exposure due to the FEL- induced mechanical stress to the cell membrane. A quantitative fluorescence microscopy is used to determine the efficiency of delivery. The result showed that the fluorescence intensity of sample cells were higher than that of control cells, and there was significant difference between the control and the sample group. Blebbing and the colony formation of the cells were observed for cells with mechanical stress.",
author = "Kunio Awazu and Seiji Ogino and Eiichi Nishimura and Takio Tomimasu and Masato Yasumoto and Jacques, {Steven L.}",
year = "1997",
language = "English (US)",
isbn = "0819423866",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
publisher = "Society of Photo-Optical Instrumentation Engineers",
pages = "302--309",
booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",
note = "Laser-Tissue Interaction VIII ; Conference date: 09-02-1997 Through 12-02-1997",
}