Delivering dye into cultured cells using infrared free-electron laser

Kunio Awazu, Seiji Ogino, Eiichi Nishimura, Takio Tomimasu, Masato Yasumoto, Steven L. Jacques

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

Free electron lasers (FELs) can be used to molecular operation such as the delivery of a number of molecules into cells. Cultured NIH3T3 cells are exposed to high-intensity short pulse FEL. The FEL is tuned to an absorption maximum wavelength, 6.1 μm, which was measured by microscopic FTIR. A fluorescence dye in the cell suspension is more absorbed into the cell with the FEL exposure due to the FEL- induced mechanical stress to the cell membrane. A quantitative fluorescence microscopy is used to determine the efficiency of delivery. The result showed that the fluorescence intensity of sample cells were higher than that of control cells, and there was significant difference between the control and the sample group. Blebbing and the colony formation of the cells were observed for cells with mechanical stress.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSociety of Photo-Optical Instrumentation Engineers
Pages302-309
Number of pages8
ISBN (Print)0819423866
StatePublished - Dec 1 1997
Externally publishedYes
EventLaser-Tissue Interaction VIII - San Jose, CA, USA
Duration: Feb 9 1997Feb 12 1997

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume2975
ISSN (Print)0277-786X

Other

OtherLaser-Tissue Interaction VIII
CitySan Jose, CA, USA
Period2/9/972/12/97

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Awazu, K., Ogino, S., Nishimura, E., Tomimasu, T., Yasumoto, M., & Jacques, S. L. (1997). Delivering dye into cultured cells using infrared free-electron laser. In Proceedings of SPIE - The International Society for Optical Engineering (pp. 302-309). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 2975). Society of Photo-Optical Instrumentation Engineers.