Delineation of the catalytic components of the NADPH-dependent O2- generating oxidoreductase of human neutrophils

Terrence R. Green, Mary K. Wirtz, David E. Wu

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Four catalytic components of the NADPH-dependent O2- generating oxidoreductase of human neutrophils have been identified. DCIP reductase, cytochrome c reductase and a chromophore 450-455 reductase are present in phorbol myristate acetate stimulated neutrophils and absent in resting cells and phorbol myristate acetate stimulated chronic granulomatous disease cells. Quinol dehydrogenase activity has also been demonstrated in activated and resting cells. Furthermore, a chromophore absorbing in the reduced state at 450-455 nm participates in superoxide production. This chromophore is reduced by NADPH or duroquinol and is missing in cell lysates derived from a patient with chronic granulomatous disease.

Original languageEnglish (US)
Pages (from-to)873-879
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume110
Issue number3
DOIs
StatePublished - Feb 10 1983

    Fingerprint

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this