Deletions in herpes simplex virus glycoprotein D define nonessential and essential domains

Veronica Feenstra, Mojgan Hodaie, David Johnson

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Herpes simplex virus glycoprotein D (gD) is a major component of the virion envelope and infected cell membranes and is essential for virus entry into cells. We have recently shown that gD interacts with a limited number of cell surface receptors which are required for virus penetration into cells. To define domains of gD which are required for aspects of virus replication including receptor binding, deletion mutations of 5 to 14 amino acids were constructed by using oligonucleotide-directed mutagenesis. Plasmids containing mutant genes for gD were assayed for the ability to rescue a recombinant virus, F-gDβ, in which β-galactosidase sequences replace gD-coding sequences. Effects on global folding and posttranslational processing of the molecules were assessed by using a panel of monoclonal antibodies which recognize both continuous and discontinuous epitopes. A region near the amino terminus (residues 7 to 21) of gD which is recognized by monoclonal antibodies able to neutralize herpes simplex virus in the absence of complement was not essential for function. In addition, virtually all of the cytoplasmic domain of gD and an extracellular domain close to the membrane were dispensable. In contrast, deletion mutations in the central region of the molecule, save for one exception, led to alterations in global folding of the molecule and maturation of the protein was inhibited.

Original languageEnglish (US)
Pages (from-to)2096-2102
Number of pages7
JournalJournal of Virology
Volume64
Issue number5
StatePublished - 1990
Externally publishedYes

Fingerprint

herpes simplex
Simplexvirus
glycoproteins
Glycoproteins
viruses
Sequence Deletion
monoclonal antibodies
Monoclonal Antibodies
Galactosidases
Viruses
galactosidases
Virus Internalization
receptors
Cell Surface Receptors
cells
Virus Replication
virus replication
oligonucleotides
Site-Directed Mutagenesis
virion

ASJC Scopus subject areas

  • Immunology

Cite this

Deletions in herpes simplex virus glycoprotein D define nonessential and essential domains. / Feenstra, Veronica; Hodaie, Mojgan; Johnson, David.

In: Journal of Virology, Vol. 64, No. 5, 1990, p. 2096-2102.

Research output: Contribution to journalArticle

Feenstra, Veronica ; Hodaie, Mojgan ; Johnson, David. / Deletions in herpes simplex virus glycoprotein D define nonessential and essential domains. In: Journal of Virology. 1990 ; Vol. 64, No. 5. pp. 2096-2102.
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