Deletion of chromosome 17p loci in breast cancer cells detected by fluorescence in situ hybridization

K. Matsumura, A. Kallioniemi, O. Kallioniemi, L. Chen, H. S. Smith, D. Pinkel, Joe Gray, F. M. Waldman

Research output: Contribution to journalArticle

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Abstract

Allelic loss of tumor suppressor genes on chromosome 17p has been implicated in the progression of breast cancer. This is in principle detectable by fluorescence in situ hybridization if the loss occurs by deletion. In order to determine if detectable deletions occur in primary breast cancer, we used dual-color hybridization with chromosome 17 pericentromeric and region-specific DNA probes to study 19 primary breast cancers. The copy numbers of 17 centromere and 17p13.1 sequences were compared with the loss of heterozygosity (LOH) for probe YNZ22 at 17p13.3 detected by restriction fragment length polymorphism. Nine of 11 cases showing LOH also showed the major population of nuclei with a deletion. The remaining two tumors with LOH were trisomie for both the centromere and 17p13.1 cosmid. In contrast, seven of eight tumors without LOH had no deletions by fluorescence in situ hybridization. These data suggest that the dominant mechanism of allelic loss at 17p in breast cancer is a physical deletion and that analysis of deletions by fluorescence in situ hybridization is a rapid and sensitive approach to studying chromosomal aberrations.

Original languageEnglish (US)
Pages (from-to)3474-3477
Number of pages4
JournalCancer Research
Volume52
Issue number12
StatePublished - Jun 15 1992
Externally publishedYes

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Chromosome Deletion
Loss of Heterozygosity
Fluorescence In Situ Hybridization
Breast Neoplasms
Centromere
Cosmids
Chromosomes, Human, Pair 17
DNA Probes
Tumor Suppressor Genes
Chromosome Aberrations
Restriction Fragment Length Polymorphisms
Neoplasms
Color
Chromosomes
Population

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Matsumura, K., Kallioniemi, A., Kallioniemi, O., Chen, L., Smith, H. S., Pinkel, D., ... Waldman, F. M. (1992). Deletion of chromosome 17p loci in breast cancer cells detected by fluorescence in situ hybridization. Cancer Research, 52(12), 3474-3477.

Deletion of chromosome 17p loci in breast cancer cells detected by fluorescence in situ hybridization. / Matsumura, K.; Kallioniemi, A.; Kallioniemi, O.; Chen, L.; Smith, H. S.; Pinkel, D.; Gray, Joe; Waldman, F. M.

In: Cancer Research, Vol. 52, No. 12, 15.06.1992, p. 3474-3477.

Research output: Contribution to journalArticle

Matsumura, K, Kallioniemi, A, Kallioniemi, O, Chen, L, Smith, HS, Pinkel, D, Gray, J & Waldman, FM 1992, 'Deletion of chromosome 17p loci in breast cancer cells detected by fluorescence in situ hybridization', Cancer Research, vol. 52, no. 12, pp. 3474-3477.
Matsumura K, Kallioniemi A, Kallioniemi O, Chen L, Smith HS, Pinkel D et al. Deletion of chromosome 17p loci in breast cancer cells detected by fluorescence in situ hybridization. Cancer Research. 1992 Jun 15;52(12):3474-3477.
Matsumura, K. ; Kallioniemi, A. ; Kallioniemi, O. ; Chen, L. ; Smith, H. S. ; Pinkel, D. ; Gray, Joe ; Waldman, F. M. / Deletion of chromosome 17p loci in breast cancer cells detected by fluorescence in situ hybridization. In: Cancer Research. 1992 ; Vol. 52, No. 12. pp. 3474-3477.
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