Delayed replication timing leads to delayed mitotic chromosome condensation and chromosomal instability of chromosome translocations

Leslie Smith, Annemieke Plug, Mathew (Matt) Thayer

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Chromosomal rearrangements are found in virtually all types of human cancers. We show that certain chromosome translocations display a delay in mitotic chromosome condensation that is associated with a delay in the mitosis-specific phosphorylation of histone H3. This delay in mitotic condensation is preceded by a delay in both the initiation as well as the completion of chromosome replication. In addition, chromosomes with this phenotype participate in numerous secondary translocations and rearrangements. Chromosomes with this phenotype were detected in five of seven tumor-derived cell lines and in five of thirteen primary tumor samples. These data suggest that certain chromosomal rearrangements found in tumor cells cause a significant delay in replication timing of the entire chromosome that subsequently results in delayed mitotic chromosome condensation and ultimately in chromosomal instability.

Original languageEnglish (US)
Pages (from-to)13300-13305
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number23
DOIs
StatePublished - Nov 6 2001

Fingerprint

Chromosomal Instability
Chromosomes
DNA Replication Timing
Phenotype
Neoplasms
Tumor Cell Line
Mitosis
Histones
Phosphorylation

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

@article{5c4d41547a8845fc92a1197cb1089dd1,
title = "Delayed replication timing leads to delayed mitotic chromosome condensation and chromosomal instability of chromosome translocations",
abstract = "Chromosomal rearrangements are found in virtually all types of human cancers. We show that certain chromosome translocations display a delay in mitotic chromosome condensation that is associated with a delay in the mitosis-specific phosphorylation of histone H3. This delay in mitotic condensation is preceded by a delay in both the initiation as well as the completion of chromosome replication. In addition, chromosomes with this phenotype participate in numerous secondary translocations and rearrangements. Chromosomes with this phenotype were detected in five of seven tumor-derived cell lines and in five of thirteen primary tumor samples. These data suggest that certain chromosomal rearrangements found in tumor cells cause a significant delay in replication timing of the entire chromosome that subsequently results in delayed mitotic chromosome condensation and ultimately in chromosomal instability.",
author = "Leslie Smith and Annemieke Plug and Thayer, {Mathew (Matt)}",
year = "2001",
month = "11",
day = "6",
doi = "10.1073/pnas.241355098",
language = "English (US)",
volume = "98",
pages = "13300--13305",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "23",

}

TY - JOUR

T1 - Delayed replication timing leads to delayed mitotic chromosome condensation and chromosomal instability of chromosome translocations

AU - Smith, Leslie

AU - Plug, Annemieke

AU - Thayer, Mathew (Matt)

PY - 2001/11/6

Y1 - 2001/11/6

N2 - Chromosomal rearrangements are found in virtually all types of human cancers. We show that certain chromosome translocations display a delay in mitotic chromosome condensation that is associated with a delay in the mitosis-specific phosphorylation of histone H3. This delay in mitotic condensation is preceded by a delay in both the initiation as well as the completion of chromosome replication. In addition, chromosomes with this phenotype participate in numerous secondary translocations and rearrangements. Chromosomes with this phenotype were detected in five of seven tumor-derived cell lines and in five of thirteen primary tumor samples. These data suggest that certain chromosomal rearrangements found in tumor cells cause a significant delay in replication timing of the entire chromosome that subsequently results in delayed mitotic chromosome condensation and ultimately in chromosomal instability.

AB - Chromosomal rearrangements are found in virtually all types of human cancers. We show that certain chromosome translocations display a delay in mitotic chromosome condensation that is associated with a delay in the mitosis-specific phosphorylation of histone H3. This delay in mitotic condensation is preceded by a delay in both the initiation as well as the completion of chromosome replication. In addition, chromosomes with this phenotype participate in numerous secondary translocations and rearrangements. Chromosomes with this phenotype were detected in five of seven tumor-derived cell lines and in five of thirteen primary tumor samples. These data suggest that certain chromosomal rearrangements found in tumor cells cause a significant delay in replication timing of the entire chromosome that subsequently results in delayed mitotic chromosome condensation and ultimately in chromosomal instability.

UR - http://www.scopus.com/inward/record.url?scp=0035818495&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035818495&partnerID=8YFLogxK

U2 - 10.1073/pnas.241355098

DO - 10.1073/pnas.241355098

M3 - Article

C2 - 11698686

AN - SCOPUS:0035818495

VL - 98

SP - 13300

EP - 13305

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 23

ER -