Deformability cytometry: Applications in clinical cancer diagnostics

H. T.K. Tse, D. R. Gossett, A. Lee, A. Ellison, Y. Ying, R. Kulkarni, J. Rao, D. Di Carlo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

A mechanical biomarker would be an attractive label-free classifier of cells for clinical diagnostic applications. However, the adoption of this biomarker has been slow due to the limited statistical confidence and power provided by low throughput techniques. Here, we demonstrate a high-throughput (2,000 cells/second) tool employing a unique combination of inertial focusing, hydrodynamic forces, and automated image analysis. Further, we demonstrate the potential of the mechanical biomarker in a clinical cancer diagnostic role in analyzing pleural effusions and in a correlative study investigating the aggressiveness of patient-derived melanoma cell lines in relation to deformability.

Original languageEnglish (US)
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages3-5
Number of pages3
StatePublished - 2011
Externally publishedYes
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume1

Conference

Conference15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

Keywords

  • Biomarkers
  • Cell mechanics
  • Deformability cytometry
  • High-throughput
  • Inertial microfluidics
  • Single-cell

ASJC Scopus subject areas

  • Control and Systems Engineering

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