Deficient dopamine D 2 receptor function causes renal inflammation independently of high blood pressure

Yanrong Zhang, Santiago Cuevas, Laureano D. Asico, Crisanto Escano, Yu Yang, Annabelle M. Pascua, Xiaoyan Wang, John E. Jones, David Grandy, Gilbert Eisner, Pedro A. Jose, Ines Armando

    Research output: Contribution to journalArticle

    26 Citations (Scopus)

    Abstract

    Renal dopamine receptors participate in the regulation of blood pressure. Genetic factors, including polymorphisms of the dopamine D 2 receptor gene (DRD2) are associated with essential hypertension, but the mechanisms of their contribution are incompletely understood. Mice lacking Drd2 (D 2-/-) have elevated blood pressure, increased renal expression of inflammatory factors, and renal injury. We tested the hypothesis that decreased dopamine D 2 receptor (D 2R) function increases vulnerability to renal inflammation independently of blood pressure, is an immediate cause of renal injury, and contributes to the subsequent development of hypertension. In D 2-/- mice, treatment with apocynin normalized blood pressure and decreased oxidative stress, but did not affect the expression of inflammatory factors. In mouse RPTCs Drd2 silencing increased the expression of TNFα and MCP-1, while treatment with a D 2R agonist abolished the angiotensin II-induced increase in TNF-α and MCP-1. In uni-nephrectomized wild-type mice, selective Drd2 silencing by subcapsular infusion of Drd2 siRNA into the remaining kidney produced the same increase in renal cytokines/chemokines that occurs after Drd2 deletion, increased the expression of markers of renal injury, and increased blood pressure. Moreover, in mice with two intact kidneys, short-term Drd2 silencing in one kidney, leaving the other kidney undisturbed, induced inflammatory factors and markers of renal injury in the treated kidney without increasing blood pressure. Our results demonstrate that the impact of decreased D 2R function on renal inflammation is a primary effect, not necessarily associated with enhanced oxidant activity, or blood pressure; renal damage is the cause, not the result, of hypertension. Deficient renal D 2R function may be of clinical relevance since common polymorphisms of the human DRD2 gene result in decreased D 2R expression and function.

    Original languageEnglish (US)
    Article numbere38745
    JournalPLoS One
    Volume7
    Issue number6
    DOIs
    StatePublished - Jun 14 2012

    Fingerprint

    Blood pressure
    renal function
    dopamine
    hypertension
    Dopamine
    inflammation
    kidneys
    Hypertension
    Inflammation
    Kidney
    receptors
    blood pressure
    Blood Pressure
    Polymorphism
    Genes
    mice
    Oxidative stress
    Dopamine Receptors
    Chemokines
    Oxidants

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Medicine(all)

    Cite this

    Zhang, Y., Cuevas, S., Asico, L. D., Escano, C., Yang, Y., Pascua, A. M., ... Armando, I. (2012). Deficient dopamine D 2 receptor function causes renal inflammation independently of high blood pressure. PLoS One, 7(6), [e38745]. https://doi.org/10.1371/journal.pone.0038745

    Deficient dopamine D 2 receptor function causes renal inflammation independently of high blood pressure. / Zhang, Yanrong; Cuevas, Santiago; Asico, Laureano D.; Escano, Crisanto; Yang, Yu; Pascua, Annabelle M.; Wang, Xiaoyan; Jones, John E.; Grandy, David; Eisner, Gilbert; Jose, Pedro A.; Armando, Ines.

    In: PLoS One, Vol. 7, No. 6, e38745, 14.06.2012.

    Research output: Contribution to journalArticle

    Zhang, Y, Cuevas, S, Asico, LD, Escano, C, Yang, Y, Pascua, AM, Wang, X, Jones, JE, Grandy, D, Eisner, G, Jose, PA & Armando, I 2012, 'Deficient dopamine D 2 receptor function causes renal inflammation independently of high blood pressure', PLoS One, vol. 7, no. 6, e38745. https://doi.org/10.1371/journal.pone.0038745
    Zhang, Yanrong ; Cuevas, Santiago ; Asico, Laureano D. ; Escano, Crisanto ; Yang, Yu ; Pascua, Annabelle M. ; Wang, Xiaoyan ; Jones, John E. ; Grandy, David ; Eisner, Gilbert ; Jose, Pedro A. ; Armando, Ines. / Deficient dopamine D 2 receptor function causes renal inflammation independently of high blood pressure. In: PLoS One. 2012 ; Vol. 7, No. 6.
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