Deficiency in rhabdomyosarcomas of a factor required for MyoD activity and myogenesis

Stephen J. Tapscott, Mathew (Matt) Thayer, Harold Weintraub

Research output: Contribution to journalArticle

154 Citations (Scopus)

Abstract

Rhabdomyosarcoma cells express the myogenic helix-loop-helix proteins of the MyoD family but do not differentiate into skeletal muscle cells. Gel shift and transient transfection assays revealed that MyoD in the rhabdomyosarcoma cells was capable of binding DNA but was relatively nonfunctional as a transcriptional activator. Heterokaryon formation with fibroblasts resulted in the restoration of transcriptional activation by MyoD and the differentiation of the rhabdomyosarcoma cells into skeletal muscle cells. These results suggest that rhabdomyosarcomas are deficient in a factor required for MyoD activity.

Original languageEnglish (US)
Pages (from-to)1450-1453
Number of pages4
JournalScience
Volume259
Issue number5100
StatePublished - Mar 5 1993
Externally publishedYes

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MyoD Protein
Rhabdomyosarcoma
Muscle Development
Muscle Cells
Skeletal Muscle
Transcriptional Activation
Transfection
Cell Differentiation
Fibroblasts
Gels
DNA

ASJC Scopus subject areas

  • General

Cite this

Deficiency in rhabdomyosarcomas of a factor required for MyoD activity and myogenesis. / Tapscott, Stephen J.; Thayer, Mathew (Matt); Weintraub, Harold.

In: Science, Vol. 259, No. 5100, 05.03.1993, p. 1450-1453.

Research output: Contribution to journalArticle

Tapscott, SJ, Thayer, MM & Weintraub, H 1993, 'Deficiency in rhabdomyosarcomas of a factor required for MyoD activity and myogenesis', Science, vol. 259, no. 5100, pp. 1450-1453.
Tapscott, Stephen J. ; Thayer, Mathew (Matt) ; Weintraub, Harold. / Deficiency in rhabdomyosarcomas of a factor required for MyoD activity and myogenesis. In: Science. 1993 ; Vol. 259, No. 5100. pp. 1450-1453.
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