Defective corneal healing in excimer laser wounded il-1 receptor deficient mice

M. C. Kennedy, L. F. Rich, Stephen Planck, J. C. Ansel, James (Jim) Rosenbaum

Research output: Contribution to journalArticle

Abstract

Purpose. To determine whether the cytokine interleukin-1 (IL-1) plays an important role in the process of corneal wound healing. We examined corneal wound repair in mice which lack a functional interleukin-1 type 1 receptor (IL-1R1-/-) and are therefore unable to respond to IL-1. Methods. IL-1R1-/- mice were photoablated in the central cornea (1 mm diameter, mid-stromal depth) using a Summit Apex excimer laser. Corneal wound healing in receptor-deficient mice (N of 40) was compared to identically wounded groups of congenic control mice (N of 30). After predetermined healing times of 1, 3, 7, or 14 days, animals were sacrificed and eyes were photographed, enucleated, and prepared for histologie examination. Results. Epithelial closure of the excimer laser wound was completed by Day 1 in both IL-1R1-/- and control mice. However on Day 3 post wounding, stromal thicknesses in the wound area were thinner in IL-1R1 ' mice, than in controls, and remained so through Day 14. Day 7 controls had a normal stratified epithelial structure while the IL-1R1-/- mice showed a poorly defined basal layer and undifferentiated cells in the upper epithelial layers. Normal corneal topography was not restored by Day 14 in IL-1R1-/- mice with the wound surface remaining flattened or convex. Conclusions. The loss of IL-1 signaling causes a defect in comeal wound healing. The IL-1R1-/- mice appear to have a reduced ability to rebuild the ablated area of corneal stroma.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume38
Issue number4
StatePublished - 1997

Fingerprint

Excimer Lasers
Interleukin-1
Wound Healing
Wounds and Injuries
Interleukin-1 Type I Receptors
Congenic Mice
Corneal Topography
Corneal Stroma
Cornea
Cytokines
Control Groups

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Defective corneal healing in excimer laser wounded il-1 receptor deficient mice. / Kennedy, M. C.; Rich, L. F.; Planck, Stephen; Ansel, J. C.; Rosenbaum, James (Jim).

In: Investigative Ophthalmology and Visual Science, Vol. 38, No. 4, 1997.

Research output: Contribution to journalArticle

@article{64c6c749867349ae9eeab556c3f5db1c,
title = "Defective corneal healing in excimer laser wounded il-1 receptor deficient mice",
abstract = "Purpose. To determine whether the cytokine interleukin-1 (IL-1) plays an important role in the process of corneal wound healing. We examined corneal wound repair in mice which lack a functional interleukin-1 type 1 receptor (IL-1R1-/-) and are therefore unable to respond to IL-1. Methods. IL-1R1-/- mice were photoablated in the central cornea (1 mm diameter, mid-stromal depth) using a Summit Apex excimer laser. Corneal wound healing in receptor-deficient mice (N of 40) was compared to identically wounded groups of congenic control mice (N of 30). After predetermined healing times of 1, 3, 7, or 14 days, animals were sacrificed and eyes were photographed, enucleated, and prepared for histologie examination. Results. Epithelial closure of the excimer laser wound was completed by Day 1 in both IL-1R1-/- and control mice. However on Day 3 post wounding, stromal thicknesses in the wound area were thinner in IL-1R1 ' mice, than in controls, and remained so through Day 14. Day 7 controls had a normal stratified epithelial structure while the IL-1R1-/- mice showed a poorly defined basal layer and undifferentiated cells in the upper epithelial layers. Normal corneal topography was not restored by Day 14 in IL-1R1-/- mice with the wound surface remaining flattened or convex. Conclusions. The loss of IL-1 signaling causes a defect in comeal wound healing. The IL-1R1-/- mice appear to have a reduced ability to rebuild the ablated area of corneal stroma.",
author = "Kennedy, {M. C.} and Rich, {L. F.} and Stephen Planck and Ansel, {J. C.} and Rosenbaum, {James (Jim)}",
year = "1997",
language = "English (US)",
volume = "38",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "4",

}

TY - JOUR

T1 - Defective corneal healing in excimer laser wounded il-1 receptor deficient mice

AU - Kennedy, M. C.

AU - Rich, L. F.

AU - Planck, Stephen

AU - Ansel, J. C.

AU - Rosenbaum, James (Jim)

PY - 1997

Y1 - 1997

N2 - Purpose. To determine whether the cytokine interleukin-1 (IL-1) plays an important role in the process of corneal wound healing. We examined corneal wound repair in mice which lack a functional interleukin-1 type 1 receptor (IL-1R1-/-) and are therefore unable to respond to IL-1. Methods. IL-1R1-/- mice were photoablated in the central cornea (1 mm diameter, mid-stromal depth) using a Summit Apex excimer laser. Corneal wound healing in receptor-deficient mice (N of 40) was compared to identically wounded groups of congenic control mice (N of 30). After predetermined healing times of 1, 3, 7, or 14 days, animals were sacrificed and eyes were photographed, enucleated, and prepared for histologie examination. Results. Epithelial closure of the excimer laser wound was completed by Day 1 in both IL-1R1-/- and control mice. However on Day 3 post wounding, stromal thicknesses in the wound area were thinner in IL-1R1 ' mice, than in controls, and remained so through Day 14. Day 7 controls had a normal stratified epithelial structure while the IL-1R1-/- mice showed a poorly defined basal layer and undifferentiated cells in the upper epithelial layers. Normal corneal topography was not restored by Day 14 in IL-1R1-/- mice with the wound surface remaining flattened or convex. Conclusions. The loss of IL-1 signaling causes a defect in comeal wound healing. The IL-1R1-/- mice appear to have a reduced ability to rebuild the ablated area of corneal stroma.

AB - Purpose. To determine whether the cytokine interleukin-1 (IL-1) plays an important role in the process of corneal wound healing. We examined corneal wound repair in mice which lack a functional interleukin-1 type 1 receptor (IL-1R1-/-) and are therefore unable to respond to IL-1. Methods. IL-1R1-/- mice were photoablated in the central cornea (1 mm diameter, mid-stromal depth) using a Summit Apex excimer laser. Corneal wound healing in receptor-deficient mice (N of 40) was compared to identically wounded groups of congenic control mice (N of 30). After predetermined healing times of 1, 3, 7, or 14 days, animals were sacrificed and eyes were photographed, enucleated, and prepared for histologie examination. Results. Epithelial closure of the excimer laser wound was completed by Day 1 in both IL-1R1-/- and control mice. However on Day 3 post wounding, stromal thicknesses in the wound area were thinner in IL-1R1 ' mice, than in controls, and remained so through Day 14. Day 7 controls had a normal stratified epithelial structure while the IL-1R1-/- mice showed a poorly defined basal layer and undifferentiated cells in the upper epithelial layers. Normal corneal topography was not restored by Day 14 in IL-1R1-/- mice with the wound surface remaining flattened or convex. Conclusions. The loss of IL-1 signaling causes a defect in comeal wound healing. The IL-1R1-/- mice appear to have a reduced ability to rebuild the ablated area of corneal stroma.

UR - http://www.scopus.com/inward/record.url?scp=33749220236&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749220236&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33749220236

VL - 38

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 4

ER -