Deep cytoplasmic rearrangements during early development in Xenopus laevis

Michael Danilchik, J. M. Denegre

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The egg of the frog Xenopus is cylindrically symmetrical about its animal-vegetal axis before fertilization. Midway through the first cell cycle, the yolky subcortical cytoplasm rotates 30° relative to the cortex and plasma membrane, usually toward the side of the sperm entry point. Dorsal embryonic structures always develop on the side away from which the cytoplasm moves. Details of the deep cytoplasmic movements associated with the cortical rotation were studied in eggs vitally stained during oogenesis with a yolk platelet-specific fluorescent dye. During the first cell cycle, eggs labelled in this way develop a complicated swirl of cytoplasm in the animal hemisphere. This pattern is most prominent on the side away from which the vegetal yolk moves, and thus correlates in position with the prospective dorsal side of the embryo. Although the pattern is initially most evident near the egg's equator or marginal zone, extensive rearrangements associated with cleavage furrowing (cytoplasmic ingression) relocate portions of the swirl to vegetal blastomeres on the prospective dorsal side.

Original languageEnglish (US)
Pages (from-to)845-856
Number of pages12
JournalDevelopment
Volume111
Issue number4
StatePublished - Apr 1991
Externally publishedYes

Fingerprint

Xenopus laevis
Cytoplasm
Eggs
Cell Cycle
Embryonic Structures
Blastomeres
Oogenesis
Xenopus
Fluorescent Dyes
Fertilization
Anura
Ovum
Spermatozoa
Blood Platelets
Cell Membrane

Keywords

  • Cleavage
  • Cytoplasmic localization
  • Dorsal-ventral axis
  • First cell cycle
  • Ingression
  • Xenopus

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Deep cytoplasmic rearrangements during early development in Xenopus laevis. / Danilchik, Michael; Denegre, J. M.

In: Development, Vol. 111, No. 4, 04.1991, p. 845-856.

Research output: Contribution to journalArticle

@article{758c0291c70c4f05bb1ba78cbd99cf5d,
title = "Deep cytoplasmic rearrangements during early development in Xenopus laevis",
abstract = "The egg of the frog Xenopus is cylindrically symmetrical about its animal-vegetal axis before fertilization. Midway through the first cell cycle, the yolky subcortical cytoplasm rotates 30° relative to the cortex and plasma membrane, usually toward the side of the sperm entry point. Dorsal embryonic structures always develop on the side away from which the cytoplasm moves. Details of the deep cytoplasmic movements associated with the cortical rotation were studied in eggs vitally stained during oogenesis with a yolk platelet-specific fluorescent dye. During the first cell cycle, eggs labelled in this way develop a complicated swirl of cytoplasm in the animal hemisphere. This pattern is most prominent on the side away from which the vegetal yolk moves, and thus correlates in position with the prospective dorsal side of the embryo. Although the pattern is initially most evident near the egg's equator or marginal zone, extensive rearrangements associated with cleavage furrowing (cytoplasmic ingression) relocate portions of the swirl to vegetal blastomeres on the prospective dorsal side.",
keywords = "Cleavage, Cytoplasmic localization, Dorsal-ventral axis, First cell cycle, Ingression, Xenopus",
author = "Michael Danilchik and Denegre, {J. M.}",
year = "1991",
month = "4",
language = "English (US)",
volume = "111",
pages = "845--856",
journal = "Development (Cambridge)",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "4",

}

TY - JOUR

T1 - Deep cytoplasmic rearrangements during early development in Xenopus laevis

AU - Danilchik, Michael

AU - Denegre, J. M.

PY - 1991/4

Y1 - 1991/4

N2 - The egg of the frog Xenopus is cylindrically symmetrical about its animal-vegetal axis before fertilization. Midway through the first cell cycle, the yolky subcortical cytoplasm rotates 30° relative to the cortex and plasma membrane, usually toward the side of the sperm entry point. Dorsal embryonic structures always develop on the side away from which the cytoplasm moves. Details of the deep cytoplasmic movements associated with the cortical rotation were studied in eggs vitally stained during oogenesis with a yolk platelet-specific fluorescent dye. During the first cell cycle, eggs labelled in this way develop a complicated swirl of cytoplasm in the animal hemisphere. This pattern is most prominent on the side away from which the vegetal yolk moves, and thus correlates in position with the prospective dorsal side of the embryo. Although the pattern is initially most evident near the egg's equator or marginal zone, extensive rearrangements associated with cleavage furrowing (cytoplasmic ingression) relocate portions of the swirl to vegetal blastomeres on the prospective dorsal side.

AB - The egg of the frog Xenopus is cylindrically symmetrical about its animal-vegetal axis before fertilization. Midway through the first cell cycle, the yolky subcortical cytoplasm rotates 30° relative to the cortex and plasma membrane, usually toward the side of the sperm entry point. Dorsal embryonic structures always develop on the side away from which the cytoplasm moves. Details of the deep cytoplasmic movements associated with the cortical rotation were studied in eggs vitally stained during oogenesis with a yolk platelet-specific fluorescent dye. During the first cell cycle, eggs labelled in this way develop a complicated swirl of cytoplasm in the animal hemisphere. This pattern is most prominent on the side away from which the vegetal yolk moves, and thus correlates in position with the prospective dorsal side of the embryo. Although the pattern is initially most evident near the egg's equator or marginal zone, extensive rearrangements associated with cleavage furrowing (cytoplasmic ingression) relocate portions of the swirl to vegetal blastomeres on the prospective dorsal side.

KW - Cleavage

KW - Cytoplasmic localization

KW - Dorsal-ventral axis

KW - First cell cycle

KW - Ingression

KW - Xenopus

UR - http://www.scopus.com/inward/record.url?scp=0025819433&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025819433&partnerID=8YFLogxK

M3 - Article

C2 - 1879356

AN - SCOPUS:0025819433

VL - 111

SP - 845

EP - 856

JO - Development (Cambridge)

JF - Development (Cambridge)

SN - 0950-1991

IS - 4

ER -