Decreased intestinal glucose transport in the sgk3-knockout mouse

Ciprian Sandu, Rexhep Rexhepaj, Florian Grahammer, James A. McCormick, Guido Henke, Monica Palmada, Srinivas Nammi, Undine Lang, Marco Metzger, Lothar Just, Thomas Skutella, Kevin Dawson, Jian Wang, David Pearce, Florian Lang

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Xenopus oocyte coexpression experiments revealed the capacity of the serum- and glucocorticoid-inducible kinase isoform 3 (SGK3) to up-regulate a variety of transport systems including the sodium-dependent glucose transporter SGLT1. The present study explored the functional significance of SGK3-dependent regulation of intestinal transport. To this end, experiments were performed in gene targeted mice lacking functional sgk3 (sgk3-/-) and their wild type littermates (sgk3+/+). Oral food intake and fecal dry weight were significantly larger in sgk3-/- than in sgk3+/+ mice. Glucose-induced current (Ig) in Ussing chamber as a measure of Na+ coupled glucose transport was significantly smaller in sgk3 -/- than in sgk3+/+ mouse jejunal segments. Fasting plasma glucose concentrations were significantly lower in sgk3-/- than in sgk3+/+ mice. Intestinal electrogenic transport of phenylalanine, cysteine, glutamine and proline were not significantly different between sgk3-/- and sgk3+/+ mice. In conclusion, SGK3 is required for adequate intestinal Na+ coupled glucose transport and impaired glucose absorption may contribute to delayed growth and decreased plasma glucose concentrations of SGK3 deficient mice. The hypoglycemia might lead to enhanced food intake to compensate for impaired intestinal absorption.

Original languageEnglish (US)
Pages (from-to)437-444
Number of pages8
JournalPflugers Archiv European Journal of Physiology
Volume451
Issue number3
DOIs
StatePublished - Dec 1 2005

Keywords

  • Colon
  • EnaC
  • Intestinal absorption
  • Knockout mouse
  • SGLT 1

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

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    Sandu, C., Rexhepaj, R., Grahammer, F., McCormick, J. A., Henke, G., Palmada, M., Nammi, S., Lang, U., Metzger, M., Just, L., Skutella, T., Dawson, K., Wang, J., Pearce, D., & Lang, F. (2005). Decreased intestinal glucose transport in the sgk3-knockout mouse. Pflugers Archiv European Journal of Physiology, 451(3), 437-444. https://doi.org/10.1007/s00424-005-1474-7