Decreased dopamine D4 receptor expression increases extracellular glutamate and alters its regulation in mouse striatum

Theresa Currier Thomas, David Grandy, Greg A. Gerhardt, Paul E A Glaser

    Research output: Contribution to journalArticle

    31 Citations (Scopus)

    Abstract

    To better understand the effect of the dopamine D4 receptor (DRD4) on glutamate (Glu) neurotransmission in the brain, we utilized transgenic mice with partial or complete removal of functional DRD4 plasma membrane expression (DRD4+/- and DRD4-/-, respectively). We measured resting extracellular Glu levels, Glu clearance kinetics, and KCl-evoked release of Glu in the striatum and nucleus accumbens core of these mice using in vivo amperometry coupled to a novel microelectrode array configured for sub-second detection of Glu. Recordings from DRD4-/- and DRD4+/- mice were compared with their wild-type littermates (DRD4+/+). Resting extracellular levels of Glu were increased in the striatum of DRD4-/- mice (p

    Original languageEnglish (US)
    Pages (from-to)436-445
    Number of pages10
    JournalNeuropsychopharmacology
    Volume34
    Issue number2
    DOIs
    StatePublished - Jan 2009

    Fingerprint

    Dopamine D4 Receptors
    Glutamic Acid
    Nucleus Accumbens
    Microelectrodes
    Synaptic Transmission
    Transgenic Mice
    Cell Membrane
    Brain

    Keywords

    • Amperometry
    • Attention-deficit/hyperactivity disorder (ADHD)
    • Dopamine D4 receptor
    • Glutamate
    • Nucleus accumbens
    • Striatum

    ASJC Scopus subject areas

    • Pharmacology
    • Psychiatry and Mental health

    Cite this

    Decreased dopamine D4 receptor expression increases extracellular glutamate and alters its regulation in mouse striatum. / Thomas, Theresa Currier; Grandy, David; Gerhardt, Greg A.; Glaser, Paul E A.

    In: Neuropsychopharmacology, Vol. 34, No. 2, 01.2009, p. 436-445.

    Research output: Contribution to journalArticle

    Thomas, Theresa Currier ; Grandy, David ; Gerhardt, Greg A. ; Glaser, Paul E A. / Decreased dopamine D4 receptor expression increases extracellular glutamate and alters its regulation in mouse striatum. In: Neuropsychopharmacology. 2009 ; Vol. 34, No. 2. pp. 436-445.
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