Decreased calcium pump adenosine triphosphatase in red blood cells of hypertensive subjects

Frank F. Vincenzi, Cynthia D. Morris, Laura B. Kinsel, Margaret Kenny, David A. McCarron

Research output: Contribution to journalArticle

75 Scopus citations

Abstract

Several operationally defined adenosine triphosphatase (ATPase) activities were determined in vitro in red blood cell lysates of normotensive or hypertensive humans: Mg2+-ATPase, Na+, K+-ATPase, and Ca2+ pump ATPase, the latter in the calmodulin-activated and basal states. Basal Ca2+ pump ATPase was defined as the Ca1+-activated ATPase resistant tol(HM trifluoperazine. Subjects were part of a double-blind study in which treatment was divided into several phases: baseline (4 weeks), placebo or calcium (1 g elemental calcium/day, 8 weeks), placebo washout (4 weeks), placebo or calcium (1 g elemental calcium/day, 8 weeks). Irrespective of the phase of treatment, the basal Ca2+ pump ATPase activity in red blood cell lysates of 36 hypertensive subjects was significantly less than that in lysates from 18 normotensive subjects. Other ATPase activities did not differ significantly, although all ATPases tended to be decreased in hypertension. The data are consistent with previous reports of altered membrane Ca2+ binding and transport in hypertension, but the precise changes are not elucidated.

Original languageEnglish (US)
Article number00020
Pages (from-to)1058-1066
Number of pages9
JournalHypertension
Volume8
Issue number11
DOIs
StatePublished - Nov 1986

Keywords

  • Calmodulin
  • Plasma membrane
  • Trifluoperazine

ASJC Scopus subject areas

  • Internal Medicine

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