Dec1 and Dec2 Expression Is Disrupted in the Suprachiasmatic Nuclei of Clock Mutant Mice

Matthew Preston Butler, Sato Honma, Tatsuya Fukumoto, Takeshi Kawamoto, Katsumi Fujimoto, Mitsuhide Noshiro, Yukio Kato, Ken Ichi Honma

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

DEC1 and DEC2 are basic helix-loop-helix transcription factors that functionally resemble negative feedback components of the mammalian circadian clock. The genes Dec1 and Dec2 are expressed rhythmically in the rat suprachiasmatic nuclei, and Dec1 expression is stimulated by light in a time-dependent manner with the kinetics of an immediate early gene. DEC1 and DEC2 can inhibit CLOCK:BMAL1 transactivation of the clock gene Per1, suggesting that these transcription factors may help regulate circadian timing. The authors present data on the expression pattern of Dec1 and Dec2 in wild-type and homozygous Clock mutant mice. In the suprachiasmatic nuclei, the Clock mutation significantly reduces the expression of Dec1 and Dec2. Dec1 becomes arrhythmic; Dec2 remains weakly rhythmic in a 12L:12D light-dark cycle but is arrhythmic in constant darkness. A robust attenuation of the Dec1 and Dec2 signals in Clock mutant mice was detected in all brain areas examined. These data point to up-regulation of Dec1 and Dec2 by Clock in vivo.

Original languageEnglish (US)
Pages (from-to)126-134
Number of pages9
JournalJournal of biological rhythms
Volume19
Issue number2
DOIs
StatePublished - Apr 2004

Keywords

  • Circadian rhythms
  • Clock genes
  • Parietal cortex
  • Per1
  • Piriform cortex
  • bHLH transcription factors

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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  • Cite this

    Butler, M. P., Honma, S., Fukumoto, T., Kawamoto, T., Fujimoto, K., Noshiro, M., Kato, Y., & Honma, K. I. (2004). Dec1 and Dec2 Expression Is Disrupted in the Suprachiasmatic Nuclei of Clock Mutant Mice. Journal of biological rhythms, 19(2), 126-134. https://doi.org/10.1177/0748730403262870