Death-Associated Protein Kinase Promoter Hypermethylation in Normal Human Lymphocytes

Ashok N. Reddy, Wei W. Jiang, Michael Kim, Nicole Benoit, Rodney Taylor, John Clinger, David Sidransky, Joseph A. Califano

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

A high frequency of death-associated protein kinase (DAPK) promoter hypermethylation has been noted in B-cell malignancies, head and neck cancers, and other solid tumors, and it has been used as a tumor marker in molecular detection strategies. Low levels of DAPK promoter hypermethylation, ranging from 0.003 to 1.181%, were detected in peripheral blood cells from 75 of 143 (52%) normal subjects by quantitative methylation-specific PCR (Q-MSP). In 10 of 10 selected patients, MSP amplification of a portion of the DAPK promoter followed by PCR product sequencing confirmed dense hypermethylation of the CpG island in their peripheral blood cells. Q-MSP analysis of fluorescence-activated cell-sorted peripheral blood cells from three of these patients demonstrated that a significantly greater proportion of B cells (1.074-6.026%) were DAPK hypermethylated than were T cells, monocytes, or neutrophils, which were

Original languageEnglish (US)
Pages (from-to)7694-7698
Number of pages5
JournalCancer Research
Volume63
Issue number22
StatePublished - Nov 15 2003
Externally publishedYes

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Death-Associated Protein Kinases
Lymphocytes
Blood Cells
Polymerase Chain Reaction
Methylation
B-Lymphocytes
CpG Islands
Tumor Biomarkers
Head and Neck Neoplasms
Monocytes
Neoplasms
Neutrophils
Fluorescence
T-Lymphocytes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Reddy, A. N., Jiang, W. W., Kim, M., Benoit, N., Taylor, R., Clinger, J., ... Califano, J. A. (2003). Death-Associated Protein Kinase Promoter Hypermethylation in Normal Human Lymphocytes. Cancer Research, 63(22), 7694-7698.

Death-Associated Protein Kinase Promoter Hypermethylation in Normal Human Lymphocytes. / Reddy, Ashok N.; Jiang, Wei W.; Kim, Michael; Benoit, Nicole; Taylor, Rodney; Clinger, John; Sidransky, David; Califano, Joseph A.

In: Cancer Research, Vol. 63, No. 22, 15.11.2003, p. 7694-7698.

Research output: Contribution to journalArticle

Reddy, AN, Jiang, WW, Kim, M, Benoit, N, Taylor, R, Clinger, J, Sidransky, D & Califano, JA 2003, 'Death-Associated Protein Kinase Promoter Hypermethylation in Normal Human Lymphocytes', Cancer Research, vol. 63, no. 22, pp. 7694-7698.
Reddy AN, Jiang WW, Kim M, Benoit N, Taylor R, Clinger J et al. Death-Associated Protein Kinase Promoter Hypermethylation in Normal Human Lymphocytes. Cancer Research. 2003 Nov 15;63(22):7694-7698.
Reddy, Ashok N. ; Jiang, Wei W. ; Kim, Michael ; Benoit, Nicole ; Taylor, Rodney ; Clinger, John ; Sidransky, David ; Califano, Joseph A. / Death-Associated Protein Kinase Promoter Hypermethylation in Normal Human Lymphocytes. In: Cancer Research. 2003 ; Vol. 63, No. 22. pp. 7694-7698.
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