Database of diazotrophs in global ocean: Abundance, biomass and nitrogen fixation rates

Y. W. Luo, S. C. Doney, L. A. Anderson, M. Benavides, I. Berman-Frank, A. Bode, S. Bonnet, K. H. Boström, D. Böttjer, D. G. Capone, E. J. Carpenter, Y. L. Chen, M. J. Church, J. E. Dore, L. I. Falcón, A. Fernández, R. A. Foster, K. Furuya, F. Gómez, K. GundersenA. M. Hynes, D. M. Karl, S. Kitajima, R. J. Langlois, J. Laroche, R. M. Letelier, E. Maranõn, D. J. McGillicuddy, P. H. Moisander, C. M. Moore, B. Mourinõ-Carballido, M. R. Mulholland, Joseph Needoba, K. M. Orcutt, A. J. Poulton, E. Rahav, P. Raimbault, A. P. Rees, L. Riemann, T. Shiozaki, A. Subramaniam, T. Tyrrell, K. A. Turk-Kubo, M. Varela, T. A. Villareal, E. A. Webb, A. E. White, J. Wu, J. P. Zehr

Research output: Contribution to journalArticle

179 Citations (Scopus)

Abstract

Marine N2 fixing microorganisms, termed diazotrophs, are a key functional group in marine pelagic ecosystems. The biological fixation of dinitrogen (N2) to bioavailable nitrogen provides an important new source of nitrogen for pelagic marine ecosystems and influences primary productivity and organic matter export to the deep ocean. As one of a series of efforts to collect biomass and rates specific to different phytoplankton functional groups, we have constructed a database on diazotrophic organisms in the global pelagic upper ocean by compiling about 12 000 direct field measurements of cyanobacterial diazotroph abundances (based on microscopic cell counts or qPCR assays targeting the nifH genes) and N2 fixation rates. Biomass conversion factors are estimated based on cell sizes to convert abundance data to diazotrophic biomass. The database is limited spatially, lacking large regions of the ocean especially in the Indian Ocean. The data are approximately log-normal distributed, and large variances exist in most sub-databases with non-zero values differing 5 to 8 orders of magnitude. Reporting the geometric mean and the range of one geometric standard error below and above the geometric mean, the pelagic N2 fixation rate in the global ocean is estimated to be 62 (52-73) Tg N yr-1 and the pelagic diazotrophic biomass in the global ocean is estimated to be 2.1 (1.4-3.1) Tg C from cell counts and to 89 (43-150) Tg C from nifH-based abundances. Reporting the arithmetic mean and one standard error instead, these three global estimates are 140 9.2 Tg N yr-1, 18 1.8 Tg C and 590 70 Tg C, respectively. Uncertainties related to biomass conversion factors can change the estimate of geometric mean pelagic diazotrophic biomass in the global ocean by about 70%. It was recently established that the most commonly applied method used to measure N2 fixation has underestimated the true rates. As a result, one can expect that future rate measurements will shift the mean N2 fixation rate upward and may result in significantly higher estimates for the global N2 fixation. The evolving database can nevertheless be used to study spatial and temporal distributions and variations of marine N2 fixation, to validate geochemical estimates and to parameterize and validate biogeochemical models, keeping in mind that future rate measurements may rise in the future.

Original languageEnglish (US)
Pages (from-to)47-73
Number of pages27
JournalEarth System Science Data
Volume4
Issue number1
DOIs
StatePublished - Aug 31 2012

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global ocean
nitrogen fixation
fixation
biomass
pelagic ecosystem
marine ecosystem
functional group
nitrogen
ocean
upper ocean
rate
temporal distribution
targeting
temporal variation
microorganism
phytoplankton
assay
spatial distribution
organic matter
productivity

ASJC Scopus subject areas

  • Earth and Planetary Sciences(all)

Cite this

Luo, Y. W., Doney, S. C., Anderson, L. A., Benavides, M., Berman-Frank, I., Bode, A., ... Zehr, J. P. (2012). Database of diazotrophs in global ocean: Abundance, biomass and nitrogen fixation rates. Earth System Science Data, 4(1), 47-73. https://doi.org/10.5194/essd-4-47-2012

Database of diazotrophs in global ocean : Abundance, biomass and nitrogen fixation rates. / Luo, Y. W.; Doney, S. C.; Anderson, L. A.; Benavides, M.; Berman-Frank, I.; Bode, A.; Bonnet, S.; Boström, K. H.; Böttjer, D.; Capone, D. G.; Carpenter, E. J.; Chen, Y. L.; Church, M. J.; Dore, J. E.; Falcón, L. I.; Fernández, A.; Foster, R. A.; Furuya, K.; Gómez, F.; Gundersen, K.; Hynes, A. M.; Karl, D. M.; Kitajima, S.; Langlois, R. J.; Laroche, J.; Letelier, R. M.; Maranõn, E.; McGillicuddy, D. J.; Moisander, P. H.; Moore, C. M.; Mourinõ-Carballido, B.; Mulholland, M. R.; Needoba, Joseph; Orcutt, K. M.; Poulton, A. J.; Rahav, E.; Raimbault, P.; Rees, A. P.; Riemann, L.; Shiozaki, T.; Subramaniam, A.; Tyrrell, T.; Turk-Kubo, K. A.; Varela, M.; Villareal, T. A.; Webb, E. A.; White, A. E.; Wu, J.; Zehr, J. P.

In: Earth System Science Data, Vol. 4, No. 1, 31.08.2012, p. 47-73.

Research output: Contribution to journalArticle

Luo, YW, Doney, SC, Anderson, LA, Benavides, M, Berman-Frank, I, Bode, A, Bonnet, S, Boström, KH, Böttjer, D, Capone, DG, Carpenter, EJ, Chen, YL, Church, MJ, Dore, JE, Falcón, LI, Fernández, A, Foster, RA, Furuya, K, Gómez, F, Gundersen, K, Hynes, AM, Karl, DM, Kitajima, S, Langlois, RJ, Laroche, J, Letelier, RM, Maranõn, E, McGillicuddy, DJ, Moisander, PH, Moore, CM, Mourinõ-Carballido, B, Mulholland, MR, Needoba, J, Orcutt, KM, Poulton, AJ, Rahav, E, Raimbault, P, Rees, AP, Riemann, L, Shiozaki, T, Subramaniam, A, Tyrrell, T, Turk-Kubo, KA, Varela, M, Villareal, TA, Webb, EA, White, AE, Wu, J & Zehr, JP 2012, 'Database of diazotrophs in global ocean: Abundance, biomass and nitrogen fixation rates', Earth System Science Data, vol. 4, no. 1, pp. 47-73. https://doi.org/10.5194/essd-4-47-2012
Luo YW, Doney SC, Anderson LA, Benavides M, Berman-Frank I, Bode A et al. Database of diazotrophs in global ocean: Abundance, biomass and nitrogen fixation rates. Earth System Science Data. 2012 Aug 31;4(1):47-73. https://doi.org/10.5194/essd-4-47-2012
Luo, Y. W. ; Doney, S. C. ; Anderson, L. A. ; Benavides, M. ; Berman-Frank, I. ; Bode, A. ; Bonnet, S. ; Boström, K. H. ; Böttjer, D. ; Capone, D. G. ; Carpenter, E. J. ; Chen, Y. L. ; Church, M. J. ; Dore, J. E. ; Falcón, L. I. ; Fernández, A. ; Foster, R. A. ; Furuya, K. ; Gómez, F. ; Gundersen, K. ; Hynes, A. M. ; Karl, D. M. ; Kitajima, S. ; Langlois, R. J. ; Laroche, J. ; Letelier, R. M. ; Maranõn, E. ; McGillicuddy, D. J. ; Moisander, P. H. ; Moore, C. M. ; Mourinõ-Carballido, B. ; Mulholland, M. R. ; Needoba, Joseph ; Orcutt, K. M. ; Poulton, A. J. ; Rahav, E. ; Raimbault, P. ; Rees, A. P. ; Riemann, L. ; Shiozaki, T. ; Subramaniam, A. ; Tyrrell, T. ; Turk-Kubo, K. A. ; Varela, M. ; Villareal, T. A. ; Webb, E. A. ; White, A. E. ; Wu, J. ; Zehr, J. P. / Database of diazotrophs in global ocean : Abundance, biomass and nitrogen fixation rates. In: Earth System Science Data. 2012 ; Vol. 4, No. 1. pp. 47-73.
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abstract = "Marine N2 fixing microorganisms, termed diazotrophs, are a key functional group in marine pelagic ecosystems. The biological fixation of dinitrogen (N2) to bioavailable nitrogen provides an important new source of nitrogen for pelagic marine ecosystems and influences primary productivity and organic matter export to the deep ocean. As one of a series of efforts to collect biomass and rates specific to different phytoplankton functional groups, we have constructed a database on diazotrophic organisms in the global pelagic upper ocean by compiling about 12 000 direct field measurements of cyanobacterial diazotroph abundances (based on microscopic cell counts or qPCR assays targeting the nifH genes) and N2 fixation rates. Biomass conversion factors are estimated based on cell sizes to convert abundance data to diazotrophic biomass. The database is limited spatially, lacking large regions of the ocean especially in the Indian Ocean. The data are approximately log-normal distributed, and large variances exist in most sub-databases with non-zero values differing 5 to 8 orders of magnitude. Reporting the geometric mean and the range of one geometric standard error below and above the geometric mean, the pelagic N2 fixation rate in the global ocean is estimated to be 62 (52-73) Tg N yr-1 and the pelagic diazotrophic biomass in the global ocean is estimated to be 2.1 (1.4-3.1) Tg C from cell counts and to 89 (43-150) Tg C from nifH-based abundances. Reporting the arithmetic mean and one standard error instead, these three global estimates are 140 9.2 Tg N yr-1, 18 1.8 Tg C and 590 70 Tg C, respectively. Uncertainties related to biomass conversion factors can change the estimate of geometric mean pelagic diazotrophic biomass in the global ocean by about 70{\%}. It was recently established that the most commonly applied method used to measure N2 fixation has underestimated the true rates. As a result, one can expect that future rate measurements will shift the mean N2 fixation rate upward and may result in significantly higher estimates for the global N2 fixation. The evolving database can nevertheless be used to study spatial and temporal distributions and variations of marine N2 fixation, to validate geochemical estimates and to parameterize and validate biogeochemical models, keeping in mind that future rate measurements may rise in the future.",
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T1 - Database of diazotrophs in global ocean

T2 - Abundance, biomass and nitrogen fixation rates

AU - Luo, Y. W.

AU - Doney, S. C.

AU - Anderson, L. A.

AU - Benavides, M.

AU - Berman-Frank, I.

AU - Bode, A.

AU - Bonnet, S.

AU - Boström, K. H.

AU - Böttjer, D.

AU - Capone, D. G.

AU - Carpenter, E. J.

AU - Chen, Y. L.

AU - Church, M. J.

AU - Dore, J. E.

AU - Falcón, L. I.

AU - Fernández, A.

AU - Foster, R. A.

AU - Furuya, K.

AU - Gómez, F.

AU - Gundersen, K.

AU - Hynes, A. M.

AU - Karl, D. M.

AU - Kitajima, S.

AU - Langlois, R. J.

AU - Laroche, J.

AU - Letelier, R. M.

AU - Maranõn, E.

AU - McGillicuddy, D. J.

AU - Moisander, P. H.

AU - Moore, C. M.

AU - Mourinõ-Carballido, B.

AU - Mulholland, M. R.

AU - Needoba, Joseph

AU - Orcutt, K. M.

AU - Poulton, A. J.

AU - Rahav, E.

AU - Raimbault, P.

AU - Rees, A. P.

AU - Riemann, L.

AU - Shiozaki, T.

AU - Subramaniam, A.

AU - Tyrrell, T.

AU - Turk-Kubo, K. A.

AU - Varela, M.

AU - Villareal, T. A.

AU - Webb, E. A.

AU - White, A. E.

AU - Wu, J.

AU - Zehr, J. P.

PY - 2012/8/31

Y1 - 2012/8/31

N2 - Marine N2 fixing microorganisms, termed diazotrophs, are a key functional group in marine pelagic ecosystems. The biological fixation of dinitrogen (N2) to bioavailable nitrogen provides an important new source of nitrogen for pelagic marine ecosystems and influences primary productivity and organic matter export to the deep ocean. As one of a series of efforts to collect biomass and rates specific to different phytoplankton functional groups, we have constructed a database on diazotrophic organisms in the global pelagic upper ocean by compiling about 12 000 direct field measurements of cyanobacterial diazotroph abundances (based on microscopic cell counts or qPCR assays targeting the nifH genes) and N2 fixation rates. Biomass conversion factors are estimated based on cell sizes to convert abundance data to diazotrophic biomass. The database is limited spatially, lacking large regions of the ocean especially in the Indian Ocean. The data are approximately log-normal distributed, and large variances exist in most sub-databases with non-zero values differing 5 to 8 orders of magnitude. Reporting the geometric mean and the range of one geometric standard error below and above the geometric mean, the pelagic N2 fixation rate in the global ocean is estimated to be 62 (52-73) Tg N yr-1 and the pelagic diazotrophic biomass in the global ocean is estimated to be 2.1 (1.4-3.1) Tg C from cell counts and to 89 (43-150) Tg C from nifH-based abundances. Reporting the arithmetic mean and one standard error instead, these three global estimates are 140 9.2 Tg N yr-1, 18 1.8 Tg C and 590 70 Tg C, respectively. Uncertainties related to biomass conversion factors can change the estimate of geometric mean pelagic diazotrophic biomass in the global ocean by about 70%. It was recently established that the most commonly applied method used to measure N2 fixation has underestimated the true rates. As a result, one can expect that future rate measurements will shift the mean N2 fixation rate upward and may result in significantly higher estimates for the global N2 fixation. The evolving database can nevertheless be used to study spatial and temporal distributions and variations of marine N2 fixation, to validate geochemical estimates and to parameterize and validate biogeochemical models, keeping in mind that future rate measurements may rise in the future.

AB - Marine N2 fixing microorganisms, termed diazotrophs, are a key functional group in marine pelagic ecosystems. The biological fixation of dinitrogen (N2) to bioavailable nitrogen provides an important new source of nitrogen for pelagic marine ecosystems and influences primary productivity and organic matter export to the deep ocean. As one of a series of efforts to collect biomass and rates specific to different phytoplankton functional groups, we have constructed a database on diazotrophic organisms in the global pelagic upper ocean by compiling about 12 000 direct field measurements of cyanobacterial diazotroph abundances (based on microscopic cell counts or qPCR assays targeting the nifH genes) and N2 fixation rates. Biomass conversion factors are estimated based on cell sizes to convert abundance data to diazotrophic biomass. The database is limited spatially, lacking large regions of the ocean especially in the Indian Ocean. The data are approximately log-normal distributed, and large variances exist in most sub-databases with non-zero values differing 5 to 8 orders of magnitude. Reporting the geometric mean and the range of one geometric standard error below and above the geometric mean, the pelagic N2 fixation rate in the global ocean is estimated to be 62 (52-73) Tg N yr-1 and the pelagic diazotrophic biomass in the global ocean is estimated to be 2.1 (1.4-3.1) Tg C from cell counts and to 89 (43-150) Tg C from nifH-based abundances. Reporting the arithmetic mean and one standard error instead, these three global estimates are 140 9.2 Tg N yr-1, 18 1.8 Tg C and 590 70 Tg C, respectively. Uncertainties related to biomass conversion factors can change the estimate of geometric mean pelagic diazotrophic biomass in the global ocean by about 70%. It was recently established that the most commonly applied method used to measure N2 fixation has underestimated the true rates. As a result, one can expect that future rate measurements will shift the mean N2 fixation rate upward and may result in significantly higher estimates for the global N2 fixation. The evolving database can nevertheless be used to study spatial and temporal distributions and variations of marine N2 fixation, to validate geochemical estimates and to parameterize and validate biogeochemical models, keeping in mind that future rate measurements may rise in the future.

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