Daily Rhythm in Plasma N-acetyltryptamine

Peter S. Backlund, Henryk Urbanski, Mark A. Doll, David W. Hein, Marjan Bozinoski, Christopher E. Mason, Steven L. Coon, David C. Klein

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Normal physiology undergoes 24-h changes in function that include daily rhythms in circulating hormones, most notably melatonin and cortical steroids. This study focused on N-acetyltryptamine, a little-studied melatonin receptor mixed agonist-antagonist and the likely evolutionary precursor of melatonin. The central issue addressed was whether N-acetyltryptamine is physiologically present in the circulation. N-acetyltryptamine was detected by LC-MS/MS in daytime plasma of 3 different mammals in subnanomolar levels (mean ± SEM: rat, 0.29 ± 0.05 nM, n = 5; rhesus macaque, 0.54 ± 0.24 nM, n = 4; human, 0.03 ± 0.01 nM, n = 32). Analysis of 24-h blood collections from rhesus macaques revealed a nocturnal increase in plasma N-acetyltryptamine (p < 0.001), which varied from 2- to 15-fold over daytime levels among the 4 animals studied. Related RNA sequencing studies indicated that the transcript encoding the tryptamine acetylating enzyme arylalkylamine N-acetyltransferase (AANAT) is expressed at similar levels in the rhesus pineal gland and retina, thereby indicating that either tissue could contribute to circulating N-acetyltryptamine. The evidence that N-acetyltryptamine is a physiological component of mammalian blood and exhibits a daily rhythm, together with known effects as a melatonin receptor mixed agonist-antagonist, shifts the status of N-acetyltryptamine from pharmacological tool to candidate for a physiological role. This provides a new opportunity to extend our understanding of 24-h biology.

    Original languageEnglish (US)
    Pages (from-to)195-211
    Number of pages17
    JournalJournal of Biological Rhythms
    Volume32
    Issue number3
    DOIs
    StatePublished - Jun 1 2017

    Fingerprint

    Melatonin Receptors
    Melatonin
    Macaca mulatta
    Arylalkylamine N-Acetyltransferase
    RNA Sequence Analysis
    Pineal Gland
    N-acetyltryptamine
    Retina
    Mammals
    Steroids
    Hormones
    Pharmacology
    Enzymes
    tryptamine

    Keywords

    • daily rhythm
    • human
    • melatonin
    • N-acetyltryptamine
    • nonhuman primate
    • pineal
    • retina
    • transcriptome profiling

    ASJC Scopus subject areas

    • Physiology
    • Physiology (medical)

    Cite this

    Backlund, P. S., Urbanski, H., Doll, M. A., Hein, D. W., Bozinoski, M., Mason, C. E., ... Klein, D. C. (2017). Daily Rhythm in Plasma N-acetyltryptamine. Journal of Biological Rhythms, 32(3), 195-211. https://doi.org/10.1177/0748730417700458

    Daily Rhythm in Plasma N-acetyltryptamine. / Backlund, Peter S.; Urbanski, Henryk; Doll, Mark A.; Hein, David W.; Bozinoski, Marjan; Mason, Christopher E.; Coon, Steven L.; Klein, David C.

    In: Journal of Biological Rhythms, Vol. 32, No. 3, 01.06.2017, p. 195-211.

    Research output: Contribution to journalArticle

    Backlund, PS, Urbanski, H, Doll, MA, Hein, DW, Bozinoski, M, Mason, CE, Coon, SL & Klein, DC 2017, 'Daily Rhythm in Plasma N-acetyltryptamine', Journal of Biological Rhythms, vol. 32, no. 3, pp. 195-211. https://doi.org/10.1177/0748730417700458
    Backlund PS, Urbanski H, Doll MA, Hein DW, Bozinoski M, Mason CE et al. Daily Rhythm in Plasma N-acetyltryptamine. Journal of Biological Rhythms. 2017 Jun 1;32(3):195-211. https://doi.org/10.1177/0748730417700458
    Backlund, Peter S. ; Urbanski, Henryk ; Doll, Mark A. ; Hein, David W. ; Bozinoski, Marjan ; Mason, Christopher E. ; Coon, Steven L. ; Klein, David C. / Daily Rhythm in Plasma N-acetyltryptamine. In: Journal of Biological Rhythms. 2017 ; Vol. 32, No. 3. pp. 195-211.
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