Cytotoxicity of resin composites containing bioactive glass fillers

Satin Salehi, Fernanda Gwinner, John C. Mitchell, Carmem Pfeifer, Jack Ferracane

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Objective To determine the in vitro cytotoxicity of dental composites containing bioactive glass fillers. Methods Dental composites (50:50 Bis-GMA/TEGDMA resin: 72.5 wt% filler, 67.5%Sr-glass and 5% OX50) containing different concentrations (0, 5, 10 and 15 wt%) of two sol-gel bioactive glasses, BAG65 (65 mole% SiO2, 31 mole% CaO, 4 mole% P2O5) and BAG61 (3 mole% F added) were evaluated for cytotoxicity using Alamar Blue assay. First, composite extracts were obtained from 7 day incubations of composites in cell culture medium at 37 °C. Undifferentiated pulp cells (OD-21) were exposed to dilutions of the original extracts for 3, 5, and 7 days. Then freshly cured composite disks were incubated with OD-21 cells (n = 5) for 2 days. Subsequently, fresh composite disks were incubated in culture medium at 37 °C for 7 days, and then the extracted disks were incubated with OD-21 cells for 2 days. Finally, fresh composites disks were light cured for 3, 5, and 20 s and incubated with OD-21 cells (n = 5) for 1, 3, 5, and 7 days. To verify that the three different curing modes produced different levels of degree of conversion (DC), the DC of each composite was determined by FTIR. Groups (n = 5) were compared with ANOVA/Tukey's (α ≤ 0.05). Results Extracts from all composites significantly reduced cell viability until a dilution of 1:8 or lower, where the extract became equal to the control. All freshly-cured composites showed significantly reduced cell viability at two days. However, no reduction in cell viability was observed for any composite that had been previously soaked in media before exposure to the cells. Composites with reduced DC (3 s vs. 20 s cure), as verified by FTIR, showed significantly reduced cell viability. Significance The results show that the composites, independent of composition, had equivalent potency in terms of reducing the viability of the cells in culture. Soaking the composites for 7 days before exposing them to the cells suggested that the "toxic" components had been extracted and the materials were no longer cytotoxic. The results demonstrate that the cytotoxicity of composites with and without BAG must predominantly be attributed to the release of residual monomers, and not to the presence of the BAG.

Original languageEnglish (US)
Pages (from-to)195-203
Number of pages9
JournalDental Materials
Volume31
Issue number2
DOIs
StatePublished - Feb 1 2015

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Bioactive glass
Composite Resins
Cytotoxicity
Glass
Fillers
Resins
Cell Survival
Composite materials
phosphorus pentoxide
Fourier Transform Infrared Spectroscopy
Culture Media
Cells
Tooth
Cell Culture Techniques
Bisphenol A-Glycidyl Methacrylate
Dental composites
Poisons
Polymethyl Methacrylate
Cell culture
Dilution

Keywords

  • Bioactive glass
  • Cytotoxicity
  • Degree of Conversion
  • Dental composites
  • FTIR
  • Undifferentiated pulp cells (OD-21)

ASJC Scopus subject areas

  • Dentistry(all)
  • Materials Science(all)
  • Mechanics of Materials

Cite this

Cytotoxicity of resin composites containing bioactive glass fillers. / Salehi, Satin; Gwinner, Fernanda; Mitchell, John C.; Pfeifer, Carmem; Ferracane, Jack.

In: Dental Materials, Vol. 31, No. 2, 01.02.2015, p. 195-203.

Research output: Contribution to journalArticle

Salehi, Satin ; Gwinner, Fernanda ; Mitchell, John C. ; Pfeifer, Carmem ; Ferracane, Jack. / Cytotoxicity of resin composites containing bioactive glass fillers. In: Dental Materials. 2015 ; Vol. 31, No. 2. pp. 195-203.
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N2 - Objective To determine the in vitro cytotoxicity of dental composites containing bioactive glass fillers. Methods Dental composites (50:50 Bis-GMA/TEGDMA resin: 72.5 wt% filler, 67.5%Sr-glass and 5% OX50) containing different concentrations (0, 5, 10 and 15 wt%) of two sol-gel bioactive glasses, BAG65 (65 mole% SiO2, 31 mole% CaO, 4 mole% P2O5) and BAG61 (3 mole% F added) were evaluated for cytotoxicity using Alamar Blue assay. First, composite extracts were obtained from 7 day incubations of composites in cell culture medium at 37 °C. Undifferentiated pulp cells (OD-21) were exposed to dilutions of the original extracts for 3, 5, and 7 days. Then freshly cured composite disks were incubated with OD-21 cells (n = 5) for 2 days. Subsequently, fresh composite disks were incubated in culture medium at 37 °C for 7 days, and then the extracted disks were incubated with OD-21 cells for 2 days. Finally, fresh composites disks were light cured for 3, 5, and 20 s and incubated with OD-21 cells (n = 5) for 1, 3, 5, and 7 days. To verify that the three different curing modes produced different levels of degree of conversion (DC), the DC of each composite was determined by FTIR. Groups (n = 5) were compared with ANOVA/Tukey's (α ≤ 0.05). Results Extracts from all composites significantly reduced cell viability until a dilution of 1:8 or lower, where the extract became equal to the control. All freshly-cured composites showed significantly reduced cell viability at two days. However, no reduction in cell viability was observed for any composite that had been previously soaked in media before exposure to the cells. Composites with reduced DC (3 s vs. 20 s cure), as verified by FTIR, showed significantly reduced cell viability. Significance The results show that the composites, independent of composition, had equivalent potency in terms of reducing the viability of the cells in culture. Soaking the composites for 7 days before exposing them to the cells suggested that the "toxic" components had been extracted and the materials were no longer cytotoxic. The results demonstrate that the cytotoxicity of composites with and without BAG must predominantly be attributed to the release of residual monomers, and not to the presence of the BAG.

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