Cytotoxic flow-cytometric crossmatches (flow-tox)

A comparison with conventional cytotoxicity crossmatch techniques

Paula J. Wetzsteon, Mary Ann Head, Lori M. Fletcher, Wai Choong Lye, Douglas Norman

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Detection and avoidance of donor-reactive antibodies in the sera of potential organ transplant recipients is key to a successful transplant outcome. Techniques of antibody detection that use flow cytometry are more sensitive than those that rely upon a visual determination of cytotoxicity. However, as conventionally performed, flow-cytometric crossmatches do not distinguish between cytotoxic (complement fixing) and noncytotoxic antibodies because both types of antibodies can bind to a cell and be detected by laser-activated fluorochrome photon emission. In 1989 we described two techniques for detecting cytotoxic antibodies using flow-cytometric techniques [1]. In 1990, we expanded the application of these new techniques that we called flow cytotoxicity assays or "Flow-Tox" [2]. Flow-Tox crossmatches demonstrate an increase in both sensitivity and specificity over conventional cytotoxicity crossmatches.

Original languageEnglish (US)
Pages (from-to)93-99
Number of pages7
JournalHuman Immunology
Volume35
Issue number2
DOIs
StatePublished - 1992

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Antibodies
Transplants
Fluorescent Dyes
Photons
Flow Cytometry
Lasers
Tissue Donors
Sensitivity and Specificity
Serum
Transplant Recipients

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Cytotoxic flow-cytometric crossmatches (flow-tox) : A comparison with conventional cytotoxicity crossmatch techniques. / Wetzsteon, Paula J.; Head, Mary Ann; Fletcher, Lori M.; Lye, Wai Choong; Norman, Douglas.

In: Human Immunology, Vol. 35, No. 2, 1992, p. 93-99.

Research output: Contribution to journalArticle

Wetzsteon, Paula J. ; Head, Mary Ann ; Fletcher, Lori M. ; Lye, Wai Choong ; Norman, Douglas. / Cytotoxic flow-cytometric crossmatches (flow-tox) : A comparison with conventional cytotoxicity crossmatch techniques. In: Human Immunology. 1992 ; Vol. 35, No. 2. pp. 93-99.
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