Cytoskeletal microdifferentiation: A mechanism for organizing morphological plasticity in dendrites

Stefanie Kaech-Petrie, Hema Parmar, Martijn Roelandse, Caroline Bornmann, Andrew Matus

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

Experimental evidence suggests that microfilaments and microtubules play contrasting roles in regulating the balance between motility and stability in neuronal structures. Actin-containing microfilaments are associated with structural plasticity, both during development when their dynamic activity drives the exploratory activity of growth cones and after circuit formation when the actin-rich dendritic spines of excitatory synapses retain a capacity for rapid changes in morphology. By contrast, microtubules predominate in axonal and dendritic processes, which appear to be morphologically relatively more stable. To compare the cytoplasmic distributions and dynamics of microfilaments and microtubules we made time-lapse recordings of actin or the microtubule-associated protein 2 tagged with green fluorescent protein in neurons growing in dispersed culture or in tissue slices from transgenic mice. The results complement existing evidence indicating that the high concentrations of actin present in dendritic spines is a specialization for morphological plasticity. By contrast, microtubule-associated protein 2 is limited to the shafts of dendrites where time-lapse recordings show little evidence for dynamic activity. A parallel exists between the partitioning of microfilaments and microtubules in motile and stable domains of growing processes during development and between dendrite shafts and spines at excitatory synapses in established neuronal circuits. These data thus suggest a mechanism, conserved through development and adulthood, in which the differential dynamics of actin and microtubules determine the plasticity of neuronal structures.

Original languageEnglish (US)
Pages (from-to)7086-7092
Number of pages7
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number13
DOIs
StatePublished - Jun 19 2001
Externally publishedYes

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Dendrites
Microtubules
Actin Cytoskeleton
Actins
Dendritic Spines
Microtubule-Associated Proteins
Synapses
Growth Cones
Neuronal Plasticity
Green Fluorescent Proteins
Transgenic Mice
Spine
Neurons

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Cytoskeletal microdifferentiation : A mechanism for organizing morphological plasticity in dendrites. / Kaech-Petrie, Stefanie; Parmar, Hema; Roelandse, Martijn; Bornmann, Caroline; Matus, Andrew.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 13, 19.06.2001, p. 7086-7092.

Research output: Contribution to journalArticle

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