Cytoskeletal changes during neurogenesis in cultures of avian neural crest cells

Melissa Haendel, Kathryn E. Bollinger, Peter W. Baas

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Neural crest cells are motile and mitotic, whereas their neuronal derivatives are terminally post-mitotic and consist of stationary cell body from which processes grow. The present study documents changes in the cytoskeleton that occur during neurogenesis in cultures of avian neural crest cells. The undifferentiated neural crest cells contain dense bundles of actin filaments throughout their cytoplasm, and a splayed array of microtubules attached to the centrosome. In newly differentiating neurons, the actin bundles are disrupted and most of the remaining actin filaments are reorganized into a cortical layer underlying the plasma membrane of the cell body and processes. Microtubules are more abundant in newly-differentiating neurons than in the undifferentiated cells, and individual microtubules can be seen dissociated from the centrosome. Neuron-specific β-III tubulin appears in some crest cells prior to cessation of motility and cell division, and expression increases with total microtubule levels during neurogenesis. To investigate how these early cytoskeletal changes might contribute to alterations in morphology during neurogenesis, we have disrupted the cytoskeleton with pharmacologic agents. Microfilament disruption by cytochalasin immediately arrests the movement of neural crest cells and causes them to round-up, but does not significantly change the morphology of the immature neurons. Microtubule depolymerization by nocodazole slows the movement of undifferentiated cells and causes retraction of processes extended by the immature neurons. These results suggest that changes in the actin and microtubule arrays within neural crest cells govern distinct aspects of their morphogenesis into neurons.

Original languageEnglish (US)
Pages (from-to)289-301
Number of pages13
JournalJournal of Neurocytology
Volume25
Issue number4
StatePublished - 1996
Externally publishedYes

Fingerprint

Neural Crest
Neurogenesis
Microtubules
Neurons
Actin Cytoskeleton
Centrosome
Cytoskeleton
Actins
Cytochalasins
Nocodazole
Tubulin
Plasma Cells
Morphogenesis
Cell Division
Cell Movement
Cytoplasm
Cell Membrane

ASJC Scopus subject areas

  • Neuroscience(all)
  • Anatomy
  • Cell Biology
  • Histology

Cite this

Cytoskeletal changes during neurogenesis in cultures of avian neural crest cells. / Haendel, Melissa; Bollinger, Kathryn E.; Baas, Peter W.

In: Journal of Neurocytology, Vol. 25, No. 4, 1996, p. 289-301.

Research output: Contribution to journalArticle

Haendel, Melissa ; Bollinger, Kathryn E. ; Baas, Peter W. / Cytoskeletal changes during neurogenesis in cultures of avian neural crest cells. In: Journal of Neurocytology. 1996 ; Vol. 25, No. 4. pp. 289-301.
@article{0e99b2be3bcb4ff1b155ef07e1e04d99,
title = "Cytoskeletal changes during neurogenesis in cultures of avian neural crest cells",
abstract = "Neural crest cells are motile and mitotic, whereas their neuronal derivatives are terminally post-mitotic and consist of stationary cell body from which processes grow. The present study documents changes in the cytoskeleton that occur during neurogenesis in cultures of avian neural crest cells. The undifferentiated neural crest cells contain dense bundles of actin filaments throughout their cytoplasm, and a splayed array of microtubules attached to the centrosome. In newly differentiating neurons, the actin bundles are disrupted and most of the remaining actin filaments are reorganized into a cortical layer underlying the plasma membrane of the cell body and processes. Microtubules are more abundant in newly-differentiating neurons than in the undifferentiated cells, and individual microtubules can be seen dissociated from the centrosome. Neuron-specific β-III tubulin appears in some crest cells prior to cessation of motility and cell division, and expression increases with total microtubule levels during neurogenesis. To investigate how these early cytoskeletal changes might contribute to alterations in morphology during neurogenesis, we have disrupted the cytoskeleton with pharmacologic agents. Microfilament disruption by cytochalasin immediately arrests the movement of neural crest cells and causes them to round-up, but does not significantly change the morphology of the immature neurons. Microtubule depolymerization by nocodazole slows the movement of undifferentiated cells and causes retraction of processes extended by the immature neurons. These results suggest that changes in the actin and microtubule arrays within neural crest cells govern distinct aspects of their morphogenesis into neurons.",
author = "Melissa Haendel and Bollinger, {Kathryn E.} and Baas, {Peter W.}",
year = "1996",
language = "English (US)",
volume = "25",
pages = "289--301",
journal = "Journal of Neurocytology",
issn = "0300-4864",
publisher = "Kluwer Academic Publishers",
number = "4",

}

TY - JOUR

T1 - Cytoskeletal changes during neurogenesis in cultures of avian neural crest cells

AU - Haendel, Melissa

AU - Bollinger, Kathryn E.

AU - Baas, Peter W.

PY - 1996

Y1 - 1996

N2 - Neural crest cells are motile and mitotic, whereas their neuronal derivatives are terminally post-mitotic and consist of stationary cell body from which processes grow. The present study documents changes in the cytoskeleton that occur during neurogenesis in cultures of avian neural crest cells. The undifferentiated neural crest cells contain dense bundles of actin filaments throughout their cytoplasm, and a splayed array of microtubules attached to the centrosome. In newly differentiating neurons, the actin bundles are disrupted and most of the remaining actin filaments are reorganized into a cortical layer underlying the plasma membrane of the cell body and processes. Microtubules are more abundant in newly-differentiating neurons than in the undifferentiated cells, and individual microtubules can be seen dissociated from the centrosome. Neuron-specific β-III tubulin appears in some crest cells prior to cessation of motility and cell division, and expression increases with total microtubule levels during neurogenesis. To investigate how these early cytoskeletal changes might contribute to alterations in morphology during neurogenesis, we have disrupted the cytoskeleton with pharmacologic agents. Microfilament disruption by cytochalasin immediately arrests the movement of neural crest cells and causes them to round-up, but does not significantly change the morphology of the immature neurons. Microtubule depolymerization by nocodazole slows the movement of undifferentiated cells and causes retraction of processes extended by the immature neurons. These results suggest that changes in the actin and microtubule arrays within neural crest cells govern distinct aspects of their morphogenesis into neurons.

AB - Neural crest cells are motile and mitotic, whereas their neuronal derivatives are terminally post-mitotic and consist of stationary cell body from which processes grow. The present study documents changes in the cytoskeleton that occur during neurogenesis in cultures of avian neural crest cells. The undifferentiated neural crest cells contain dense bundles of actin filaments throughout their cytoplasm, and a splayed array of microtubules attached to the centrosome. In newly differentiating neurons, the actin bundles are disrupted and most of the remaining actin filaments are reorganized into a cortical layer underlying the plasma membrane of the cell body and processes. Microtubules are more abundant in newly-differentiating neurons than in the undifferentiated cells, and individual microtubules can be seen dissociated from the centrosome. Neuron-specific β-III tubulin appears in some crest cells prior to cessation of motility and cell division, and expression increases with total microtubule levels during neurogenesis. To investigate how these early cytoskeletal changes might contribute to alterations in morphology during neurogenesis, we have disrupted the cytoskeleton with pharmacologic agents. Microfilament disruption by cytochalasin immediately arrests the movement of neural crest cells and causes them to round-up, but does not significantly change the morphology of the immature neurons. Microtubule depolymerization by nocodazole slows the movement of undifferentiated cells and causes retraction of processes extended by the immature neurons. These results suggest that changes in the actin and microtubule arrays within neural crest cells govern distinct aspects of their morphogenesis into neurons.

UR - http://www.scopus.com/inward/record.url?scp=0030010735&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030010735&partnerID=8YFLogxK

M3 - Article

C2 - 8793733

AN - SCOPUS:0030010735

VL - 25

SP - 289

EP - 301

JO - Journal of Neurocytology

JF - Journal of Neurocytology

SN - 0300-4864

IS - 4

ER -